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20 Cards in this Set

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What should platelet estimations be described as?

adequate, increased, or decreased.

What do giant platelets indicate?

early bone marrow release

what is a normal platelet count for a dog?

200,000-500,000/ µl

What is a normal platelet count for a feline?

300,000-700,000/ µl

define thrombocytopenia

deficiency of platelets in the blood. This causes bleeding into the tissues, bruising, and slow blood clotting after injury.

What are platelets?

They are small, discoid anuclear cells found in circulation and are derived from the cytoplasm of megakaryocytes in the bone marrow .

the purpose of any quality control program is to monitor ------ and ------

accuracy and precision

what is the unopette system?

The Unopette System is designed for the collection and preparation of micro blood specimens for hematology and chemistry. The disposable tests are unitized providing fresh pre-measured reagents. The Unopette System standardizes pipetting and dilution improving the precision of laboratory testing

what is the difference between preanalytic variables and analytic variables and how they would affect your results?

Preanalytic variable factors may be biologic or non biologic. Since they can’t be controlled they must be considered by the veterinarian when evaluating test results. Analytic variables affect the procedure because its measured by the instrument. The impact on a test result will differ between labs depending on the type on instrumentation.

name a time that doing a manual WBC count is valuable even in a fully automated clinic lab

A manual WBC count is indicated when verifying automated counts and also evaluating morphology.

is the platelet number seems to be low on the blood smear what would be your next step?

If platelet numbers are low on the blood smear, a more quantitative count should be done using the Unopette system. You should also examine the feathered edge for clumping.

How soon should Platelet counts should be done?
within 2 hours of blood collection.
How do you calculate the platelet count per µl of blood?
Calculation: Multiply the number of platelets counted (25 small squares of the center primary square) times 1000. .Example: you counted 322 platelets. Your platelet count is 322,000/µl
when should instruments be calibrated?

As a general rule, instruments should be recalibrated whenever new reagents are placed into service, after any electronic servicing, and any time that quality controls begin to show a drift (and all other causes have been eliminated).

where on the blood smear do you count platelets?

Platelets should be counted in the monolayer area of the smear; the number of platelets found in several oil immersion fields (OIF) should be averaged

Why should blood not be allowed to remain in anticoagulant for more than a few hours?
some platelet clumping may occur

What is a hemocytometer?

The hemocytometer or counting chamber is a specimen slide which is used to determine the concentration of cells in a liquid sample. It is frequently used to determine the concentration of blood cells.
The microhematocrit centrifuge is subject to normal wear and tear and therefore should be calibrated when?

monthly

What is considered an adequate number of platelets on the blood smear on oil immersion?
normal 8-12 platelets/oil immersion field
What is a common problem associated with stain integrity?
A common problem is artifacts which are caused by moisture.