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23 Cards in this Set
- Front
- Back
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State the 5 properties of proteins that can be measured. |
Mass Charge-pH Hydrophobic/hydrophilic properties Differential solubility Mobility in applied fields |
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Sepereation science relies on 4 main methods. State all 4 of these. |
Centrifigation Dialysis Lyophilisation Precipitation |
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Describe centrifugal force. Describe centrafugal force. |
Centrifugal force: Force feels like it's fleeing towards you, reference from outside the centre Centrafugal force: Reference from centre, feels like it's moving away |
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What formulae can be used to calculate the force of centrifugation? What can this formulae be rearranged to produced? |
F=mw^2r w=2piN/60 |
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In the above equation, what does the following correspond to: F, m, w, r, N? |
F: Force N m: Mass of particle w: Average angular velocity (rad/s) N: revolution per minute (r.p.m) |
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g=11.18 x r x(n/1000)^2 What does the above equation describe? Give both names? |
G-force of a centrifuge a.k.a Relative Centrifugal Force |
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From the flashcard above, what does: g, r and n correspond to? |
g: equivalent gravitational force (9.8 ms^-2) r: length of the rotor (cm) n: number of revolutions per minute (RPM) |
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RCF isn't uniform within a centrifuge tube. Where would the highest, and lowest, RCF values be seen? |
Highest: near outside of rotor (rmax) Lowest: near central axis (rmin) |
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What is the general relationship between RPM and RCF values? |
RPM squared is RCF |
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The centrifuge classes are low, high and ultra. What are the relative RPM (x10^3) and RCF values for each of the 3 classes? |
Low: RPM:10, RCF:7 High: RPM: 28 RCF: 100 Ultra: RPM: 100/150 RCF: 800/900 |
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What is the smallest pelleting application a low speed centrifuge can produce? How about High speed? And ultra? |
Low: Nuclei High: Precipitates and virus' Ultra: Macromolecules (All can be pelleted) |
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Density gradient centrifution allows you to seperate by properties. What are the 2 sub catagories of centrifugation found here? Give an example of each. |
Rate zonal centrifugation: sucrose Isopycnin centrifugation: CsCl |
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Define Isopycnin. |
Fluid of the same density |
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What technique must you do in the lab to ensure sample layering is done correctely? |
Add liquid down the side of the tube, uses capillary action to be accurate |
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What is a Svedberg unit? What 2 things does it depend on? What does it tell us about an object? |
Sedimentation coefficient Mass and shape It's speed in relation to its acceleration |
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Give 3 examples of dialysis membrane. |
Collodion, cellophane and cellulose |
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What is the name given when a solid turns directly into a gas? What about when a gas turns directly into a solid? |
Sublimation Deposition |
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The removal of solvent from a frozen sample is given what name? Can cause protein _ and _? |
Lyophilisation Denaturation and aggregation |
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State 3 protein protectants. |
Sugars, polyols and glocols |
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Why is seperation by percipitation not commonly used today? |
Can result in protein denaturing |
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Proteins are usually least soluble around what point? Define entropy. |
Isoelectric point Fundamental measure of disorder |
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What is the link between: Kosmotropes and Choanotropes and interactions? |
Kosmotropes: Increase the interactions order within the waterby increasing order Choanotropes: Decrease the interactions within the water, decreasing order |
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Define chaperone proteins? |
Proteins that assist the covalent folding or unfolding and the assembly or diassasembly of other macromolecular structures |
