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63 Cards in this Set

  • Front
  • Back
• Prokaryote shapes
o Cocci- Spheres
o Bacilli- rods
o Spiral
• Prokaryotic cell walls
o Maintains shape, structure
o Protection
o Prevents cell from bursting or shriveling in hyper/hypo tonic environments
o Made of cellulose or chitin
o Peptidoglycan
• In most bacterial cell walls
• Network of modified sugar polymers cross linked by short polypeptides
• Encloses entire bacterium and anchors other molecules that extend from its surface
• Gram positive bacteria
o Have simpler walls with large amount of peptidoglycan
• Gram negative bacteria
o Have less peptidoglycan
o Structurally more complex
o Has an outer membrane of lipopolysaccharides (carbs bounded to lipids)
• Lipopolysaccharides are often toxic, secrete toxins
o More deadly than gram positive bacteria
o More resistant to antibiotics b/c of strong outer membrane
• How do antibiotics work?
o They inhibit the formation of peptidoglycan (which forms the cell wall)
o Work well in gram positive bacteria
o Only destroy bacteria b/c human cells don’t have peptidoglycan
• Capsule
o Covers cell wall of prokaryotes
o Sticky layer of polysaccharide or protein
o Enables bacteria to adhere to things
o Serve as protection against immune systems
• Fimbriae and Pili
o Hair like structures that enable bacteria to adhere to substrate or other bacteria
o Fimbriae are more numerous and shorter than Pili
• Flagella and Cilia
o Flagella are most common
• Can have one or more flagella
o Prokaryote flagella are thinner and shorter than eukaryotic flagella
o Help bacteria move
• Taxis
o Prokaryotes exhibit taxis in a heterogeneous environment
o Taxis→ ability to respond to stimuli
o Bacteria will respond to stimuli
• Membrane function in prokaryotes
o Cellular membrane can preform cellular respiration, photosynthesis
• Nucleoid region
o Region where the prokaryote’s DNA is located
o Prokaryote’s DNA is located in the prokaryotic chromosome
o Bacteria have a single chromosome in the nucleoid region
o All essential functions are coded in the bacterial chromosome
• Plasmids
o Smaller rings of DNA in bacteria
o Consist of only a few genes
o Provide resistance to antibiotics, direct the metabolism of rarely encountered nutrients
o Plasmids contain optional DNA, all essential bacteria functions are coded in the prokaryotic chromosome
o Can replicate independently
o Bacteria transfer plasmids through conjugation- helps their survival by exchanging genes
• Endospores
o Certain bacteria form resistant cells called endospores when an essential nutrient is lacking in the environment
o Original cell replicates its chromosome and surrounds it with a tough wall (forming an endospore)
o Metabolism within the endospore ceases
o The rest of the cell disintegrates, leaving the endospore behind
o Endospores are so durable they can survive in boiling water
o Endospores help bacteria survive- its like hibernation for bacteria
• Phototrophs
• Phototrophs→ obtain energy from light
• Chemotrophs
• Chemotrophs→ obtain energy from chemicals
• Autotrophs
• Autotrophs→ organisms that only need CO2 to obtain carbon
• Heterotrophs
• Heterotrophs→ require an organic nutrient like glucose to obtain carbon
• Photoautotrophs
• Photoautotrophs→ photosynthetic organisms that capture light energy and use it to drive the synthesis of organic compounds from CO2
• Chemoautotrophs
• Chemoautotrophs→ need only CO2 as a carbon source, oxidize inorganic substances for energy
• Photoheterotrophs
• Photoheterotrophs→ use light for energy, use organic forms of carbon as a carbon source
• Chemoheterotrophs
• Chemoheterotrophs→ use organic molecules for both energy and for carbon
• Obligate aerobes
o Use O2 for cellular respiration and cannot grow without it
• Facultative anaerobes
o Use O2 if it is present but can also grow by fermentation in an anaerobic environment
• Obligate anaerobes
o Poisoned by O2
o Some live exclusively by fermentation
o Some live by extracting chemical energy by anaerobic respiration
• Nitrogen fixation
o Certain prokaryotes convert N2 into NH3 (ammonia)
o Cyanobacteria are nitrogen fixers
• Metabolic cooperation in bacteria
o Anabaena lives in colonies
o Some bacteria carry out only photosynthesis, some bacteria carry out only nitrogen fixation
o Intercellular connections allow bacteria to exchange carbohydrates and nitrogen with each other
o This way, the entire colony can fix nitrogen and carry out photosynthesis
• Biofilms
o Metabolic cooperation occurs in surface coating colonies known as biofilms
o Colony cells secrete signaling molecules that recruit nearby cells, causing the colony to grow
o Produce proteins that adhere the cells to the substrate and to one another
• Prokaryotic phylogeny
o Prokaryotes are extremely diverse
o Horizontal gene transfer (bacterial transfer of plasmids) is very significant in the evolution of prokaryotes
• Prokaryotes have acquired genes from distantly related species in this way
• Constant transfer of genes diversifies prokaryotes
• Archaea
o No peptidoglycan
o Has some branched hydrocarbons for membrane lipids
o Several kinds of RNA polymerase
o Methionine (start amino acid codon thing)
o Has some introns in its genes
o Doesn’t respond to antibiotics
o Has histones
o Circular chromosome
o Some species can grow at temperatures greater than 100 celsius
• Bacteria
o Has peptidoglycan
o Has unbranched hydrocarbons for membrane lipids
o One kind of RNA polymerase
o Formyl-Methionine (start amino acid codon thing)
o Doesn’t have introns in its genes
o Negatively affected by antibiotics
o No histones
o Circular chromosomes
o Can’t survive at temperatures greater than 100 celsius
• Eukarya
o Has nuclear envelope and membrane enclosed organelles
o No peptidoglycan
o Has unbranched hydrocarbons for membrane lipids
o Several kinds of RNA polymerase
o Methionine (start amino acid codon thing)
o Has introns in its genes
o Doesn’t respond to antibiotics
o Has histones
o No circular chromosomes
o Can’t survive at temperatures greater than 100 celsius
• Extremophiles
o Archaea bacteria that live in extreme conditions
o Oldest prokaryotes classified in the domain archaea
o Extreme thermophiles, halophiles, methanogens
• Extreme thermophiles
o Archaea bacteria that thrive in very hot environments
• Extreme halophiles
o Archaea bacteria that thrive in highly saline environments
o Very salty places
• Methanogens
o Use CO2 to oxidize H2 and release methane as a waste product
o Poisoned by O2
• Proteobacteria
o Large and diverse clade of gram negative bacteria
o Includes photoautotrophs, chemoautotrophs, and heterotrophs
o Either anaerobic or aerobic
o Five subgroups of proteobacteria
• Alpha, beta, gamma, delta, epsilon
• Chlamydias
o Parasites that survive only within animal cells
o Depend on host for resources like basic ATP
o Lack peptidoglycan
• Spirochetes
o Helical heterotrophs
o Some are notorious pathogenic parasites like syphilis and lyme disease
• Cyanobacteria
o Photoautotrophs
o Only prokaryotes with plant like oxygen generating photosynthesis
o Chloroplasts likely evolved from cyanobacteria
• Decomposers
o Bacteria function as decomposers
o Without decomposers, chemical cycling would cease and live would end on earth
• Symbiosis
o An ecological relationship between organisms of different species that are in direct contact
o Bacteria have symbiotic relationships with most organisms (we have E coli in our digestive tract)
• Host
• Host: the thing the symbiont lives in in a symbiotic relationship
• Mutualism
• Mutualism: both benefit from the relationship
• Commensalism
• Commensalism: one benefits while the other is not affected
• Parasitism
• Parasitism: one benefits and the other is harmed
• Exotoxins
o Proteins secreted by prokaryotes
• Endotoxin
o Toxic Lipopolysaccharide components of the outer membrane of gram negative bacteria
o Released only when the bacteria die and their cell walls break down
• Bioremediation
o Use of organisms to remove pollutants from soil air or water
• Bacteria help clean up pollution
o Prokaryotes are very useful
• Protists
o Unicellular or multicellular eukaryotes
o most nutritionally diverse: photoautotrophs, heterotrophs, mixotrophs (both heterotroph and photoautotroph)
o Very diverse: some are more closely related to plants, animals, or fungi
o Diverse habitats
• Endosymbiotic theory
o Earliest eukaryotes acquired mitochondria by engulfing a bacteria
o Organelles originated from symbiotic relationship between a eukaryote and a prokaryote
o Primary endosymbiosis: eukaryote engulfs prokaryote
o Secondary endosymbiosis: the same eukaryote engulfs another prokaryote
• Diplomonads
o No plastids
o No DNA, ETC, or enzymes in mitochondria
o Anaerobic
o Have two equal sized nuclei
o Multiple flagella
o Ex: Giardia
• Parabasalids
o Ex: T vaginalis
o No plastids
o No DNA, ETC, or enzymes in mitochondria
o Anaerobic
• Euglenozoa
o Have flagella with unique internal structure
• Has a spiral or crystalline rod of unknown function inside flagella
o 2 kinds: Kinetoplastids and Euglenids
• Kinetoplastids
o Single large mitochondrion that contains an organized mass of DNA called kinetoplast
o Free living consumers of prokaryotes in freshwater, marine, moist terrestrial ecosystems
o Flagella
• Has a spiral or crystalline rod of unknown function inside the flagella
o Ex: Trypanosoma (sleeping sickness)
• Euglenids
o Have a pocket at one end of the cell where one or two flagella emerge
o Ex: Euglena (both photosynthetic and heterotrophic)
• Alveolates
o Have membrane bounded sacs (alveoli) under plasma membrane
• Function is unknown
o two groups: dinoflagellates and ciliates
• Dinoflagellates
o Heterotrophic or phototrophic
o Most are unicellular, some are colonial
o Internal cellulose plates reinforce cell wall
o Have two flagella
o Cause red tide when they “bloom”
o Some release toxins that can poison animals
o Some are bioluminescent
• Ciliates
o Protists that use cilia to move/feed
o Have large macronuclei and small micronuclei
o Some have oral groove, use cilia to sweep food into oral groove

ex: paramecium
• Formation of cyst
o Mosquito bites person and injects protest into person
o Protist goes into person’s liver cells, replicate, burst out, and penetrate the blood cells
o Protist divides asexually in blood cells
o Protest bursts out of blood cells
o Mosquito bites person and picks up protest
o Zygotes form in mosquito’s digestive tract
o Zygotes form a cyst as they get bigger
o Cyst bursts
• Conjugation and reproduction
o Two cells align side by side and partially fuse
o Meiosis of micronuclei produces four haploid micronuclei in each cell
o Cells exchange micronuclei
o Cells separate
o One micronuclei from each cell fuse together in each cell (now you have a diploid micronucleus)
o Diploid micronucleus divides mitotically in each cell
o Original macronucleus disappears in each cell
o Four diploid micronuclei become one macronucleus, four diploid micronuclei remain micronuclei: in each cell
o Cell splits to create four daughter cells
• Conjugation
o Sexual process in which two individuals exchange haploid micronuclei
• Diatoms
o Type of Stramenopile
o Unicellular algae
o Unique glass like wall made of hydrated silica embedded in an organic matrix
o Very strong cell wall
o Asexual reproducers
o Major component of phytoplankton
• Red Algae/Green Algae
o Closet relatives to land plants
o Red algae:
• most abundant large algae in warm coastal waters
• multicellular
• red b/c of pigment phycoerythrin which masks green chlorophyll
o Green algae
• Chloroplasts
• Closely related to land plants
• Chlorophytes and charophyceans (two main groups)