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40 Cards in this Set
- Front
- Back
Jembec plate
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MTM for Neisseria gonnorheae
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Enterobacteriaceae
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GNR
Oxidase negative Ferment Glucose Reduce nitrate to nitrite |
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TSI - Salmonella typhi |
K/A no gas, H2S+/- no urea |
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Haemophilus influenzae
Haemophilus parainfluenzae Haemophilus hemolyticus |
Needs XV, -ALA
Needs V, +ALA Needs XV, +ALA, hemolysis on quad |
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Beta lactamase Test
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Nitrocefin disk (cephalosporin)
Positive: pink red Negative: no color change |
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X & V Requirements Test
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X factor: hemin
V factor: NAD |
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DNase
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Positive= clearing Staphlylococcus aureus
Negative = |
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Novobiocin susceptibility test
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S. saprophyticus = resistant = no zone of inhibition
CNS/ S. aureus = sensitive = 16 mm zone or larger |
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Staph identification tests
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Catalase, DNase, Coagulase, novobiocin, Staphurex
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Streptococcus identification tests
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Catalase, Bile esculin, sodium chloride tolerance, Camp, Spot CAMP, Optochin, Bile solubility, Bacitracin, Hippurate hydrolysis, PYR, latex
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Camp
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Positive (arrow) = Group B Strep
negative = Group A strep |
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Optochin susceptibility test (P disc)
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Selectively inhibits the growth of S. pneumoniae
Positive (zone) = Strep. pneumo negative (no zone) = Strep viridans |
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Bile solubility
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Disappearance of the tested colonies - hydrolyzed area
Positive = S. pneumoniae |
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Bacitracin susceptibility test
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Positive (zone) = group A Beta hemolytic Strep
negative (no zone) = non-Group A Beta hemolytic Strep |
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Hippurate Hydrolysis
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Enzyme hippuicase hydrolyses Na hippurate to benzoic acid and glycine
Positive (Purple) = Group B Strep, (gardnerella vaginalis, campy) Negative (clear) = Group A Strep |
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Plate CAMP Test
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Hemolytic activity of S. aureus is enhanced by extracellular protein produced by Beta Strep Group B
Arrow = Beta Strep GroupB No arrow = Beta Strep group A |
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Neisseria gonorrhoeae
Neisseria meningitidis Moraxella |
Growth on MTM/Choc
Diplococci Oxidase + |
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Throat
Urine sputum wound |
Beta Strep A
S. aureus, S. sapro, E. faecalis, Enterobacteriaceae S. pneumo, Pseudomonas, Haemophilus, Neisseria Anything |
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Gram Positive Bacilli
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Listeria monocytogenes
Erysipelothrix rhusiopathiae Corynebacterium diphtheriae Bacillus sp. Lactobacillus sp. |
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Listeria spp. tests (GPB)
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Bile - esculin
Catalase Narrow zone of hemolysis |
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Catalase + GPR
(4) |
Listeria monocytogenes
Erysipelothrix rhusiopathiae Corynebacterium diphtheriae Bacillus spp. |
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Hydrogen Sulfide Production
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H2S reacts with iron salks to produce black precipitate ferric sulfide
Erisipelothrix rhusiopathiae + (only GPR +) |
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Loeffler's Serum Medium
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Isolates C. diphtheriae but is non-selective
produces cells with metachromatic granules when stained with methylene blue stain |
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Serum Tellurite agar
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Corynebacterium dephtheriae will appear as brown-black colonies with intense halo
*selective and differential |
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Double zone of hemolysis
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Clostridium perfringens
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microscopic preparations for mycology
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Lactophenol cotton blue
Periodic acid-schiff stained smear KOH prep |
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C. albicans screen
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Positive: PRO+ and NGL+
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Germ Tube Test
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Positive: C. albicans, C. dublinensis
Negative: C. tropicalis |
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Gram neg cocci
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Neisseria (diplo)
Moraxella (cocco) Pasteurella Brucella Francicella |
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D-ALA Porphryin Test
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ALA disc
Rxn: orange red fluorescence Screens: H. influenzae, H. parainfluenzae, H. haemolyticu |
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Bacitracin
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Bacitracin disc from blood agar
Rxn: zone Screen: Beta Strep group A |
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Esculin Hydrolysis Test
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Esculin Disc
Rxn: dark, no fluorescence Screens: Enterococci +, Beta Strep group A neg |
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Rapid hippurate Test
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Hippurate disc, nihydrin reagent
Rxn: purple color Screens: Beta strep Group B, Gardnerella, Campy + |
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Na Hippurate
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Rxn: Purple color in tube
Screens: Beta Strep Group B +, Beta Strep Group A neg |
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Novobiocin Test
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Discs, 0.5 McFarland
Rxn: Zone Screens: Staph. saprophyticus +, Coag Neg Staph - neg |
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Optochin Test
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Taxo discs on Blood agar plate
Rxn: zone Screens: Strep. pneumo, Strep viridans (group A) neg |
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PYR
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Disc
Rxn: pink/red in 1 minute screens: Enterococcus faecalis +, Alpha Strep viridans neg, S. lugdunensis +, CNST - |
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Salt Tolerance
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Heart infusion broth
Rxn: growth Screens: Enterococci faecalis +, Group D neg |
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Plate CAMP test
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Blood agar, Staph aureus, beta Strep B, beta Strep A
Rxn: arrow head hemolysis Screens: Beta strep Group B +, Beta strep Group A |
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Group D |
Not entero: S. bovis Entero: E. faecalis |