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18 Cards in this Set
- Front
- Back
Restriction enzymes
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cut DNA at specific sequences -- very important for manipulating
DNA in the lab. |
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Bacteria make restriction enzymes. The recognition sites for the cleavage are either 4 or 6
____ long. |
bases
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palindromic
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DNA sequence of both strands when read in the same orientation (5' to 3') is the same
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6 base recognition site
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4096 bases 6^4
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4 base recognition site
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256 bases 4^4
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_________ will not work on methylated DNA.
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Restriction enzymes
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When bacteria are infected by a ____, the _____ DNA enters the cell but is not
methylated. The restriction enzymes can chop up the _____ DNA and prevent it from taking over the cell and turning it into a _____ factory. |
phage
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SNPs stands for:
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single nucleotide polymorphisms
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____ amplify
many regions with single nucleotide polymorphisms that affect restriction sites. |
PCR
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Agarose is derived from _____
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agar
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In an agrose gel, The _______charged DNA moves towards the ______ pole - _____ pieces move
_____ than longer ones. |
negatively ; positive; shorter ; faster
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RFLPs stand for
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Restriction Fragment Length Polymorphisms
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RFLPs can be used to identify:
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people and track genetic linkage and inheritance
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Plasmids
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small circles of double-stranded DNA - 3,000 to 10,000 bases long
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transformation
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the insertion of a plasmid into a bacterial cell
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overhangs
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can hydrogen bond together and the strands can be sealed with DNA ligase enzyme
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dideoxynucleotides are known as
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chain terminators.
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Reverse transcriptase
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makes a DNA copy of RNA
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