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11 Cards in this Set
- Front
- Back
- 3rd side (hint)
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PCR |
An in vitro technique that can rapidly amplify a target DNA sequence |
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Template DNA |
Double-stranded DNA that contains the target sequence to be amplified |
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PCR Steps |
Denaturation Annealing Extension |
3 |
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Denaturation |
PCR is heated to 94c The heat denatures the double-stranded DNA |
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Annealing |
PCR mix is cooled to 55c Allow primers to anneal to each template strand Primers flank the target sequence to be amplified |
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Extension |
PCR mix is heated to 72c Taq polymerase synthesizes a strand of DNA complementary to the template strands Extending from the 3' end of the primers |
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Transporter gene |
Gene determining eye color |
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Magnesium use |
Allows the Taq polymerase to bind |
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dNTP use |
Building blocks that base pair the new strand via Taq polymerase (N=A, C, G, T) |
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*Primers use (P1, P2, P3) |
Tells where to start DNA replication |
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Taq polymerase |
Enzyme that creates DNA |
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