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14 Cards in this Set
- Front
- Back
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What do we ofter require for detection of antibody |
An enzyme or fluorochrome |
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What can be conjugated to an antibody to allow detection |
Enzymes Radiation Fluorescencewhat |
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What can antibodies be linked chemically to |
Enzymes Fluorescent molecules Radioactive molecules enzyme beads |
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What is to detect or quantify antibodies made in response to microbial infections |
Serology |
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The type of antibody detected in a persin during infection can determine what |
Ir the person has experienced the infection Had a historic infection or a recent infection About the persons cellular immune response |
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what antibodies are associated with recent infection and historic infection |
IgM- recently acquired infection IgG-historic or chronic infection |
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Name the 5 steps for an elisa for IgG |
Coat surface with antigen Add serum (contain IgG) Add anti-IgG Add substrate Stop reaction and measure |
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Name 5 steps in a elisa for igM |
Surface coat with antigen Add serum(contains IgM) Add anti-IgM Add substrate Stop reaction and measure |
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Why are samples serially diluted |
So a end point can be determined |
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How can microbes be detected |
Directly using antibodies that have been generated to them |
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Whats an ELISA for a pathogen |
Surface coated with anti-pathogen ab Add sample (contain pathogen) Add anti-pathogen Add substrate Stop reaction and measure |
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Fluorescence is the stimulated emission of light from what |
From a substance which has absorbed radiation of another wavelength |
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Whats some comminly used fluorochromes |
Green fluorescent protein (GFP) FITC PE |
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Conjugating different fluorochromes to different antibodies allows us to detect what |
When and where antibodies are found |
