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24 Cards in this Set
- Front
- Back
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What is glycosylation |
Adding sugars to proteins |
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O-linked |
Serine, thernine Short chains Only in golgi |
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N-linked |
Asparagine Longer chains Starts in ER continues to golgi |
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Recognition sequence that’s to tell 3,9,2 sugar to go onto protein |
Asn-x-ser |
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Disulfide bonds |
Only in lumen of ER Cysteine is used |
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PDI protein disulfide isomerase |
Can create and rearrange disulfide bonds. |
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What does tunicamycin do |
Blocks 3,9,2 sugar from binding |
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Step 1 of moving soluble proteins into organelles |
Hsp 70 uses atp to keep protein unfolded |
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Step 2 of moving soluble proteins into organelles |
Matrix targeting protein binds to outer membrane TOM20/22 |
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Step 3 of moving soluble proteins into organelles |
TOM 20/22 gives protein to TOM 40 which is by the contact site |
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Step 4 of moving soluble proteins into organelles |
Protein moves through TOM40 and gets inserted into TIM 23/17 |
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Step 5 of moving soluble proteins into organelles |
Moves through TIM while being bound to Hsp70. Matrix targeting sequence is removed by matrix processing protease |
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Step 6 of moving soluble proteins into organelles |
Hsp 70 hydrolysis releases protein and is them folded into active protein |
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What has to be happening for proteins to be moved into organelles |
Has to be undergoing oxidative phosphorylation |
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Import of peroxisomal matrix proteins |
Single membrane no dna or ribosomes Comes from cytosol Proteins can be folded Targeting sequence is on C terminus and is not removed |
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GTP to GDP requires what |
GAP |
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GDP to GTP requires what |
GEF |
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Proteins to nucleus steps |
-Binding of NLS of a cargo protein to form importin-cargo complex - ICC diffuses through NPC - Ran-GDP needs to be GTP to break complex -GAP breaks complex and importin is sent to cytoplasm |
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Vesicle budding step 1 |
Step 1: recruitment of gtp binding G proteins Sar1-GDP is recruited to donor membrane by GEF sec12 Gef- guanine exchange factor converts GDP->GTP N terminus of sar 1 gets inserted into ER membrane |
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Vesicle budding step 2 |
recruits sec23/24 coat proteins •Coat proteins create curvature of membrane -What cargo proteins are gonna be loaded by integral membrane proteins Di-acidic sorting signal -Asp-X-Glu Soluble cargo has luminal sorting sequence that bind to the luminal domain. Cargo binding cargo Sec 23 acts as gap for sar 1. Sar 1 only active for certain amount of time |
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Vesicle budding step 2 |
recruits sec23/24 coat proteins •Coat proteins create curvature of membrane -What cargo proteins are gonna be loaded by integral membrane proteins Di-acidic sorting signal -Asp-X-Glu Soluble cargo has luminal sorting sequence that bind to the luminal domain. Cargo binding cargo Sec 23 acts as gap for sar 1. Sar 1 only active for certain amount of time |
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Vesicle budding step 3 |
Release of sar 1-GDP causes disassembly of coat. |
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Vesicle docking |
Initial docking is promoted by -Rab GTPases Rab-GTP binds to a rab effector protein complex. |
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Vesicle fusion |
V-snare protein forms stable coiled-coil interaction. T-snare comes from golgi. Snare complex disassembled by N-ethylmaleimide sensitive factor (NSF) and alpha-SNAP. Requires ATP. |
