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6 Cards in this Set

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1. Compare and contrast RNA and DNA.

RNA is single stranded


RNA has ribose instead of deoxyribose


RNA has uracil instead of thymine

2. What is RNA processing? Why is RNA processed?

RNA is processed so mRNA can leave the nucleus through pores.




1. 5' capping


2. Splicing


3, Polyadenylation

3. What is wobble base pairing?

The "Wobble Hypothesis,"discovered by Frances Crick, states that rules of base pairing are relaxed at the third position, so that a base. can pair with more than one complementary base.




A wobble base pair is a pairing between two nucleotides in RNA molecules that does not follow Watson-Crick base pair rules.




The four main wobble base pairs are guanine-uracil (G-U), hypoxanthine-uracil (I-U), hypoxanthine-adenine (I-A), and hypoxanthine-cytosine (I-C)




The third position of the codon is the wobble position. The wobble position is the base that changes to form the same AA most of the time (third)

4. What is an open reading frame?

An ORF is a continuous stretch of codons that do not contain a stop codon (usually UAA, UAG or UGA).

5. How do prokaryotes and eukaryotes differ during transcription and translation?

Prokaryotic- one kind of RNA polymerase for transcription, no introns, polycistronic (can encode numerous proteins), transcription and translation are coupled




Eukaryotic- 3 RNA polymerase, transcription in the nucleus, mRNA processed in the nucleus (3 steps) mRNA is exported to cytoplasm after 3 processing

Start/Stop Codons

Methionine (AUG) is a start codon


UAA, UAG, UGA are all stop codons