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17 Cards in this Set

  • Front
  • Back

Order of draw

1. Blood culture bottles


2. Blue top tubes


3. Red top tubes or SST


4. Green top tubes


5. Lavender and pink top tubes


6. Gray top tubes

Reasons to reject a specimen

Improper labelling


Specimen drawn above IV


Wrong tube


Specimen not transported to lab in timely manner


Hemolysis

Lipemic

Milky/cloudy (turbid) serum caused by elevated triglycerides



**may absorb light and alter results**

Icteric

Dark yellow/slight green serum caused by an elevated bilirubin



**interferes with albumin and total protein tests**

What veins are used for blood draw?

Cephalic, median cubital, and basilic

Why does hemolysis cause falsely increased test results?

Because blood work is only testing the serum or plasma and the bursting red blood cells add to those test results especially the falsely increased potassium

Blood received in the lab for blood gas analysis must meet what requirements?

On ice, no clots, and no air bubbles

Unless blood gas measurements are made immediately after sampling in vitro, glycolysis of the blood causes a:

Fall in pH and a rise in PCO2 because the sample will metabolize glucose

What serum constituent is unstable if a blood specimen is left standing at room temperature for 8 hours before processing?

Glucose because it decreases at a rate of 5%-7% per hour at room temperature inn whole blood. Glycolysis will continue until the specimen is processed buy centrifugation and serum/plasma it's separated from the cellular components of the blood.

An arterial blood specimen submitted for blood gas analysis was obtained at 8:30 a.m. but wasn't received in the lab until 11 a.m.. The tech should:

Request a new specimen because the first wasn't received in a timely manner.

What is the formula for calculating absorbance given the percent transmittance (%T) of a solution?

2-log(%T)

What method is susceptible to the solvent displacing effect that results in falsely decreased electrolyte values?

Indirect ion- selective electrodes because the electrolyte exclusion effect applies only to the indirect methods and is caused by the solvent displacing effect of high concentration of lipids and protein in the sample resulting in falsely decreased values.

In a specimen collected for plasma glucose analysis, sodium fluoride:

Inhibits glycolysis by exerting its preservative action that stops the enzyme systems involved in glycolysis

As part of a hyperlipidemia screening, the following results were obtained on a 25 year old female 6 hours after eating:



Triglycerides: 260mg/dL (2.86 mmol/L)


Cholesterol: 120 mg/dL (3.12 mmol/L)



What is the best way to interpret the results?

The cholesterol is normal but the triglycerides are elevated most likely due to the recent meal. A high fat diet will elevate the serum concentration of triglycerides. Fasting overnight (10-14 hours) is optimal to standardize blood collections including lipids.

Blood was collected in an SST on a payment who has been fasting since midnight. If it was collected at 7 a.m., what lab test would need to be recollected?

Triglycerides because it requires a minimum of 10 hours of fasting to get accurate results.

Blood was collected in an SST on a payment who has been fasting since midnight. If it was collected at 7 a.m., what lab test would need to be recollected?

Triglycerides because it requires a minimum of 10 hours of fasting to get accurate results.

Arterial blood that is collected in a heparinized syringe but exposed to room air would be most consistent with what changes?

PO2 elevated


PCO2 decreased


pH elevated


Because the presence/exposure of excess gas (O2) in the syringe used to collect blood gas specimens will cause diffusion of CO2 out of the specimen, O2 into the specimen, and an increase in pH