Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
25 Cards in this Set
- Front
- Back
|
Sequencing an organism's genome is NOT the only way to determine where genes are located within the genome
True of False |
True |
|
|
In Bacteria, the chromosomes are generally ____ and plasmids usually are ____.
Fill in circular and/or linear |
In bacteria, the chomosomes are generally circular and the plasmids usually are circular.
|
|
|
A plasmid vector that can integrate into a bacterium's genome is called a(n)
a. bacterial artificial chomosome (BAC) b. episome c. tRNA d. transposon |
episome |
|
|
Conjugative plasmids contain a(n) ____ region that allows for the plasmid to be transferred from one cell to another.
a. con b. mob c. ori d. tra |
tra |
|
|
Most gram-negative bacteria replicate plasmids by ____, and gram-postive bacteria often copy their plasmids by ___.
a. A similar chromosome-aplification mechanism / A similar chromosome-amplifying mechanism. b. A similar chromosome-amplifying mechanism / rolling circle amplification. c. Rolling circle amplification / a similar chromosome-amplifying mechanism d. rolling circle amplification / rolling circle amplification |
a similar chomosome-amplifying mechanism / rolling circle amplification |
|
|
Which is the LEAST abudant element in a nucleotide?
a. carbon b. hydrogen c. oxygen d. phosphorus |
phosphorus |
|
|
Which component(s) is/are present in nucleotides but lacking in nucleosides?
a. nitrogen base b. penthose sugar c. phosphate d. all of the above |
phosphate
|
|
|
The base pairing between ____ is especially strong, because it contains an additional ___ bond compared to ____.
a. A and T / ionic / C and G b. A and T / hydrogen / C and G c. C and G / ionic / A and T d. C and G / hydrogen / A and T |
C and G / hydrogen / A and T |
|
|
The enzyme responsible for coiling chromosomal DNA into several supercoiled domains is called DNA
a. gyrase b. ligase c. polymerase d. torsionase
|
gyrase
|
|
|
The three steps (in order) _____ are cycled, ofter 30 times, in PCR using a thermostable DNA polymerase to amplify a particular DNA sequence.
a. anneal, denature, and extend b. denature, anneal, and extend c. denature, extend, and anneal d. extend, anneal, and denature |
denature, anneal and extend
|
|
|
Which enzyme is NOT present in a replisome?
a. DNA helicase b. DNA gyrase c. DNA primase D. DNA polymerase III |
DNA gyrase |
|
|
Which statement below provides an explanation of why a lagging strand (rather then two leading strands) exists during DNA replication?
a. New nucleotides can only be added to a 3'(-OH) end. b. Only one primer can exist at a time. c. There is only one DNA polymerase per cell. d. There is only one replisome per cell |
New nucleotides can only be added at a 3'(-OH) end |
|
|
The location of bacterial chromosome where DNA replication begins is OriC, and the binding of _____ opens up the double helix.
fill in : DnaA or DNA gyrase |
DnaA |
|
|
What is the function of the Tus protein ( encoded by tus)?
a. Binds the terminus (Ter site) and blocks progress of the replication fork. b. Holds togehter the two core enzymes for the leading and lagging strands. c. prevents single strand of DNA from annealing. d. unlinks interlocked circles
|
Binds the terminus (Ter site) and blocks progress of the replication fork. |
|
|
Which feature is true for RNA polymerase activity but NOT true for DNA polymerase activity?
a. ATP, CTP, and GTP(guanosinetrifosfaat) are precursors. b. Growth of nucleotides sequense is only in the 5'-->3' direction. c. newly synthesized strands are antiparallel to its template strand. d. nucleotide primer is unnecessary |
nucleotide primer is unnecessary |
|
|
The sigma subunit of TNA polymerase functions to
a. form RNA polymerase - DNA complexes. b. locate, recognize, and bind to promoter regions. c. terminate mRNA synthesis of genes at their stop sites. d. Do both a and b. |
Form RNA polymerase - DNA complexed, locate, recognize, and bind to promoter regions. |
|
|
Which enzyme is responsible for opening up the dsDNA in order for RNA polymerase to transcribe a gene?
a. DNA helicase b. DNA gyrase c. RNA polymerase d. topoisomerase |
DNA helicase |
|
|
A nucleotide sequense that usually containts 5'TATAAT3' is called the
a. -35 sequence b. alternative start site c. Pribnow region d. transcriptional start site |
Pribnow region
|
|
|
Molecules that regulate the activity of sigma factors in a selective manner, such as regulating σ38 during stationary growth phase, are called
a. anti-sigma factors. b. coagulating sigma factors c. repressive sigma factors d. sigma facotrs effectors |
anti-sigma factors |
|
|
The most common first-position amino acid in bacterial proteins is
a. isoluecine b. leucine c. methionine d. N-formylmethionine |
N-formylmethionine |
|
|
During translation, a (3-nucleotide) codon in an mRNA transcript is recognized by a(n)_____ that carries with it the corresponding amino acids.
a. rRNA anticodon b. rRNA codon c. tRNA anticodon d. tRNA codon |
tRNA anticodon |
|
|
What form of energy is used during both initiation and elongation of protein synthesis?
a. cAMP b. GTP (Guanosinetrifosfaat) c. proton motive force d. rATP |
GTP |
|
|
Ribosomes are often the target of antibacterial drugs such as tetracycline
True of False |
True |
|
|
How is 16S rRNA involved in the translation of proteins?
a. it encodes for ribosomes that mediate the process. b. it fixes stalled mRNA synthesis that lack an anticodon by synthesizing the appropriate tRNA required for the ribosome to continue translating. c. it forms the structural backbone of ribosomes, but is not directly involved in translation. d. it helps initiate translation by base pairing with the Shine-Dalgarno sequence on the mRNA |
it helps initiate translation by base pairing with the Shine-Dalgarno sequence on the mRNA |
|
|
In Bacteria, the most common form of amino acids in the proteins is
a. D-enantiomers. b. L-enantiomers c. negatively charged d. of the cis arrangement |
L-enantiomers |
