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69 Cards in this Set
- Front
- Back
Kjeldahl's procedure for total protein is based upon the premise that
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the nitrogen content of proteins is constant
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the term "biuret reaction" refers to
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coordinate bond btwn Cu2+ & carboxyl and amino groups of biuret
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which statement about the biuret reaction for total protein is true
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polypetides & compounds w/ repeating imine groups react
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which of the following protein methods has the highest analytic sensitivity
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Folin lowry
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which of the following statements regarding proteins is true
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total protein & albumin are about 10% higher in ambulatory patients
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hyperalbuminemia is caused by
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dehydration syndromes
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high serum total protein but low albumin is usually see in
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multiple myeloma
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which of the following condtx is most commonly ass w/ elevated level of total protein
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malignancy
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which of the following dyes is the most specific for mz of albumin
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BCP - bromcresel purple
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which of the following factors is most likely to cause a falsely low result when using the BCG dye binding for albumin
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the presence of penicillin
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at pH 8.6 proteins are _____ charged & migrate towards the ______
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negatively
anode |
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electrophoretic movement of protein toward the anode will be made to decrease by increasing the
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ionic strength of the buffer
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at pH 8.6, the cathodal movement of gamma (Y) globulin is caused by
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electroendomosis
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which of the following condtx below will prevent any migration of protein across an electrophoretic support medium such as agarose
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loss of contact btwn a buffer chamber & the medium
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which of the proteins listed below has the highest pH
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IgG
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which of the proteins listed below migrates in the beta region at pH 8.6
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transferrin
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which of the following is one advantage of high resolution (HR) agarose electrophoresis over lower current electrophoresis
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high resolution procedures detect monoclonal & oligoclonal bands at a lower concentration
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which of the following condtx is ass w/ Beta gamma (BY) bridging
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hepatic cirrhosis
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which support medium can be used to determine the molecular weight of a protein
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polyacrylamide gel
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which of the following stains is used for lipoprotein electrophoresis
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oil red O
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which of the following serum protein electrohporesis result suggested an acute inflammatory process
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albumin - decrease
alpha 1 - increase alpha 2 - increase Beta - normal gamma - normal |
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which of the following condtx is usually ass w/ an acute inflammatory pattern
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myocardial infarction (MI)
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what is the clinical utility of testing for serum prealbumin
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serial low level indicate compromised nutritional status
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which serum protein should be measured in patient suspected of having Wilson's disease
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ceruloplasmin
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a pat w/ hemolytic uremic syndrome ass w/ septicemia has a normal haptoglobin level although the plasma free HGB is elevated & hemoglobinuria is present - which test would be more appropriate than haptoglobin to mz this pat's hemolytic episode
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hemopexin
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quantitative determination of HGB A2 is best performed by
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column chromatography
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HGB F concentration is usually mz by
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alkali denaturation of HGB A and HGB A2
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Select the correct order of HGB migration on agarose or cellulose acetate at pH 8.6
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-- C-->S-->F-->A +
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which of the following abnormal HGBs migrates to the same position as HGB S on agarose or cellulose acetate at pH 8.6
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HGB D punjab
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which HGB is a Beta-Delta chain hybrid & migrates to the same position as HGB S at pH 8.6
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HGB lepore
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select the correct order of HGB migration on CITRATE agar at pH 6.2
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-- F-->A--->S--->C +
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which HGB separates from HGB S on citrate (acid) agar but not on agarose or cellulose agar
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HGB D punjab
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in double immunodiffusion reactions the precipitin band is
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concave to the protein of greatest molecular weight
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which statement best describes immunofixation electrophoresis (IEF)
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proteins are separated by electrophoresis followed by overlay of monospecific anti-immunoglobulins
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which of the following statement regarding the identification of monoclonal proteins by IFE is true
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when testing for monoclonal gammopathy, both serum & urine must be examined
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which statement regarding IFE is true
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CSF should be concentrated 50 to 100 fold before performing IFE
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which of the following statement regarding IFE is true
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oligoclonal banding is seen in the CSF of more than 90% of multiple sclerosis cases
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detection of isoemz bands following electrophoresis is facilitated by
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adding substrates to th gel & mz catalytic activity
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which isoemz of CK has the fastest electrophoretic mobility at pH 8.6
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BB
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capillary electrophoresis differs from agarose gel elecrophoresis in which respect
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a stationary support is not used
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select the order of mobility of lipoprotein electrophoresed on cellulose actate or agarose at pH 8.6
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-- Chylomicrons-->Beta---> pre-Beta--> alpha +
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following ultracentrifugation of plasma, which fraction correlates w/ pre-B lipoprotein
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VLDL - very low density lipoprotein
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select the lipoprotein fraction that carriers most of the endogenous triglycerides
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VLDL
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the protein composition of HDL is what percentage by weight
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50%
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broad (floating) B lipoprotein occurs in familial B-dyslipoproteinemia (type III hyperlipoproteinemia) & consist of
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IDL
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which of the following mechz accounts for the elevated plasma levels of B-lipoproteins seen in familiar hypercholesterolemia (type II hyperlipoproteinemia)
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ApoB 100 receptor defect
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which emz defcy is most commonly ass w/ familial hypertriglyceridemia, which is ass w/ fasting plasma chylomicrons (type I hyperlipoproteinemia)
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postherparain activated lipoprotein lipase defncy
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which of the following condtx is most consistently ass w/ secondary hypercholesterolemia (type II lipoproteinemia)
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hypothyroidism
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which of the following is ass w/ Tangier's disease
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apoprotein A-I defncy
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what is the formula for LDL calc
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LDL chol = TC - (HDL + TG/5)
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which of the following formulas correctly estimates the BLDL cholesterol
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serum trig divided by 5
TRIG/5 |
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which is the screening procedure for adults recommended by National Cholesterol Ed Program, to eva risk for atherosclerosis
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lipid profile, fasting every 5 years
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what is the most appropriate fasting procedure when a lipid study of trig, TC, HDL & LDL tests are ordered
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12 hours, nothing but water allowed
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select the cutpoint value for serum or plasma LDL chol in a person w/ history of coronary heart dz in whom therapeutic lifestyle changes should be initiated
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>100 mg/dL
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what is the HDL chol cutpoint recommend by the NCEP
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<40 mg/dL
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an EDTA blood sample is collected from a nonfasting person for a CBC, the doc collected the sample from the femoral vein cz venipuncture from the arm was unsuccessful. he called the lab 15 mins after the sample arrive & request a lipid studying inc TRIG, TC, HDL & LDL - which test results should be used to eva the pat's risk for coronary artery disease
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TC and HDL cholesterol
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which of the following dz is caused by a deficiency of SPHINGOMYELINASE
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Niemann Picks disease
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which method is considered the candidate reference method for TRIG measurement
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CDC modification of Van Handel & Zilversmit
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which of the following emz is common to all emz methods for TRIG measurement
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glycerol kinase
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select the reagent needed in the coupling emz rtx used to generate a color product in the cholesterol oxidase method for chol
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Phenol
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which of the following method for HDL chol is the reference method
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ultracentrifugation
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cholesterol esterase is used in emz assays to
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hydrolyze fatty acids bound to the 3rd carbon atom of cholesterol
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which of the following reagents is used in the direct HDL chol method
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sulfate cyclodextrin
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what do "direct" or homogenous method for LDL chol ass have in common
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all use a detergent to facilitate selective reactivity w/ reagent emz
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what is the purpose of the saponification step used in the Abell Kendall method for chol mz
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convert chol esters to free cholesterol
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lipoproteins (a) or Lp(a) is important when elevated in serum because
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is an independent risk factor for atherosclerosis
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which type of dietary fatty acid is not ass w/ increase in serum LDL chol production
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monosaturated cis-fatty acids
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lab results shows as follow what do you do next
TC = 220 mg/dL HDL chol = 40 mg/dL TRIG = 420 mg/dL |
perform a direct LDL chol assay
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a person has a fasting TRIG of level of 240 mg/dL, the doc wishes to know the patients non HDL chol level, what chol fraction should be measured
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TC and HDL cholesterol
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