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32 Cards in this Set
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Listeria monocytogenes |
-Primary an animal pathogen -Narrow band of Beta-hemolysis
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Forms the so called |
"Inverted Christmas tree" pattern / umbrella like growth on agar surface SIM media |
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Disease |
1.) Listeriosis - Food poisoning 2.) Septicemia- can infect blood 3.) Meaning in elderly and immunocompromised |
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Mode of transmission: |
Consumption of contaminated food, particularly COLESLAW and SOFT CHEESE |
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Virulence factor: |
Listeriolysis O- hemolytic cytotoxic toxin that allows survival of organism within phagocytes |
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CAMP test (+) |
Can tolerate cold enrichment(4°c for several weeks) |
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CAMP test for Listeria uses: |
Rhodococcus equi as known organism |
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Positive result |
Block-Type hemolysis |
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2. Erysipelothrix rhusiopathiae |
H²S positive on TSI media ( it can cause blackening in large amount) ;Non motive, Catalase (+) |
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Causes |
Erysipeloid, a localized skin infection, an occupational hazard for those handing meat and poultry and fish; hemolytic pattern: can be either alpha ( usually) or beta-hemolytic |
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3. Lactobacillus acidophilus/ Doderlain Bacilli |
Produced large amount of lactic acid, non pathogenic and has little clinical significance; |
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PAP stained smear |
Can be seen in PAP stained smear since it's a normal flora: Abnormal increase of L. Acidophilus may indicate DM or patient might taking contraceptive |
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Normal flora of mouth, |
GIT and vaginal canal ( it can protect from urogenital bacteria) |
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Trivia: |
L. Acidophilus can be a component of yogurt |
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Culture Media: |
THA - Tomato juice Agar |
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Genus Corynebacterium |
4.1.Corynebacterium diphtheriae aka Klebs-Loeffler's Bacillus
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Non-existent motile, highly pleomorphic |
Can assume different forms; bacillus with granules |
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One end typically swollen |
Club shaped appearance; pallisade arrangement ( side by side arrangement). Picket fence arrangement and x, y, v, l formation ( Chinese letter arrangement) |
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With Babes Emst granules |
Monochromatic granules that act as energy storing substance (polyphosphate) |
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On BAP; |
Produce narrow zone of beta-hemolysis |
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Loeffler's serum and PAI's Coagulated Egg serum |
Enhance granule formation and pleomorphism |
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-Loeffler's serum: |
Small , moist, slightly, raised grayish colonies with entire margin ( poached-egg colony); enhance pigmentation of organism |
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- PIA Coagulated Egg Medium; |
enhances pleomorphism and granules formation; can produce Gray to black colony. |
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Cytine Tellurite Blood Agar ( CTBA); |
Modification of tinsde medium ( for primary isolation of C. Diphtheria); selective-differential media, inhibitor: Potassium Tellurite ( inhibits Grams (-) organisms (+) result: Grey to back colonies |
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Virulence Factor |
Causes diphtheria by those able to produce toxin Virulence Factor : Diptheria toxin- a very powerful exotoxin that attacks the mucous membrane of respiratory tract resulting to inflammation, following necrosis that can lead to pseudomembrane formation ( cardinal sign for diptheria infection) |
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Disease Diptheria |
It is droplet infection characterized by low grade Fever, mild sore throal and body weakness. Patient may manifest a bull-neck appearance due to enlargement lymph nodes. |
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3 Colony types on CTA |
Gravis, mitosis, intermedius; these colony types however, have nothing to do with the degree/ severity of the infection. |
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Gravis |
Largest colony type; dark grey colony with irregular edges; 1-2 mm in diameter |
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Mitis |
Fried egg appearing organism on BAP |
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Intermedius |
Small and black colonies; 0.5 mm in diameter |
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Modified ELEK test positive (+) - |
It is a toxigenicity test to determine whether the organism is a toxin producer or not. |
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Modified ELEK test positive (+) - |
On agar media, place a filter paper strip with diphtheria anti-toxin, streak + control at right angle to the trip of anti-Semitism toxin, streak negative control in the same manner. Unknown culture suspected of C. diphtheriae is streaked parallel to(+) and (-) control. Incubate at 35°C for 24-48 hours (+) result; Lines of Precipitation |