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34 Cards in this Set

  • Front
  • Back

hemoglobin is also known as

respiratory pigment


identified respiratory pigment

Felix seyler

Reference method of hemoglobin determination

cyanmethemoglobin

reagent of cyanmethemoglobin

drabkin's (potassium ferricyanide)

converts hemoglobin to methemoglobin



Potassium ferricyanide (K3Fe)

all hemoglobin is measured except

sulfhemoglobin

high wbc count correcction

centrifuge and measure supernatant

lipemia correction

0.01 patient plasma + 5 ml cyanmethemoglobin reagent

abnormal globulin correction

0.1 g potassium carbonate to drabkins reagent

method that does not produce toxic waste. Used in some automated instruments

sodium lauryl sulfate (SLS)

Slowest hemoglobin

hgb A2


hgb C


hgb E


hgb O


hgb C(harlem)

differentiates hemoglobin S,D,G, C, E

citrate agar

migrate to the same area at cellulose acetate electrophoresis

Hgb S


Hgb D


Hgb G

HEME a.k.a

ferroprotoporphyrin IX

site of heme synthesis

Mitochondria

Globin syntheis



production of A and Z globin

Chromosome 16

Globin synthesis



Production of B,E,D and G

Chromosome 11

2 zeta


2 gamma

Portland

2 alpha


2 epsilon

Gower 2

Hgb bound to oxygen


Found in arterial blood


"Relaxed state"

Oxyhemoglobin

Hgb not bound to oxygen


In venous blood


"Tense state"

Deoxygenated hemoglobin

Hgb bound to CO


240 times greater affinity


Tasteless, colorless, odourless


Carboxyhemoglobin

Mixture of oxidized, partially denatures forms of hemoglobin

Sulfhemoglobin

Prolonged constipation


Enterogenous cyanosis


Color of blood: mauve lavender

Sulfhemoglobin

Color of blood: cherry red

Carboxyhemoglobin

Cells containing HB S and C

make 1:2 dilution with distilled water and multiply by 2

Fastest hgb

Hemoglobin A1

aka heme syntetase

ferrochelatase

enzyme needed to insert Fe (ferrous) to the protophorphyin IX

ferrochelatase

2 zeta


2 epsilon

Gower 1

2 alpha


2 gamma

High F

2 alpha


2 beta

A1

2 alpha


2 delta

A2

Hb with Fe3+, not bound to O2


Color of blood: chocolate brown

Methemoglobin