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49 Cards in this Set
- Front
- Back
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Tissue should be placed in a suitable fixative immediately after removal from the body to:
a) prevent decomposition due to enzymatic activity b) permit the dehydrant to function properly c) inhibit cross-linking of tissue proteins d) stabilize tissue carbohydrates |
a) prevent decomposition due to enzymatic activity
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A fixative containing potassium dichromate:
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will make tissue more receptive to eosin staining
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A specimen is submitted with the statement that it was fixed in formalin. Microscopic sections show marked lysis of erythrocytes. This indicates that the fixative most likely was:
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acetified with acetic acid
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Microscopic examination of an H&E stained section reveals the presence of extraneous epithelial cells. This is most likely caused by:
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albumin spread on the slide by a finger
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Microscopic examination of an H&E stained section reveals the presence of irregular holes scattered throughout the section. This is most likely caused by:
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sections being taken immediately after "rough facing" of the block
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The only kidney biopsy tissue available has been fixed in phosphate buffered glutaraldehyde for 2 hours and then placed in phosphate buffer solution. If a portion of this tissue is processed for light microscopy, sections would most likely show:
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nonspecific PAS stain
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Sections of paraffin embedded tissue show straight lines that appear to be fissures or cuts running at many different angles. This artifact is most likely due to:
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use of quick-freeze spray to chill the block
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Which of the following groups of special stains requires sections cut at 8um to 10um?
a) Congo red, Lieb crystal violet, Bielchowsky b) Kinyoun, Snook, oil red O c) von Kossa, Fontana-Masson, Sudan black B d) Verhoeff-van Gieson, aldehyde fuchsin, Masson trichrome |
a) Congo red, Lieb crystal violet, Bielchowsky
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When cutting 30um sections of fixed brain tissue in a -15⁰C cryostat, the sections fragment at the knife edge. This problem could most likely be prevented by:
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raising the cryostat temperature to -5⁰C
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A section of liver is cut in the cryostat and the sections obtained are alternately thick and thin, with a distinct "venetian blind" effect. The most probable cause of this artifact is that the:
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knife is dull
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If asked to cut cryostat sections of tissue fixed in Zenker solution, the histotechnician should:
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treat the tissue with iodine, hypo, and water before freezing
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Uric acid crystals are preserved only when the tissue is fixed in:
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absolute alcohol
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Pigment caused by mercury-containing fixatives can be removed from tissues by:
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iodine-sodium thiosulfate
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The subsequent demonstration of chromaffin granules requires which of the following fixatives?
a) orth b) bouin c) B-5 d) formalin |
a) Orth
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Improper preservation of tissue will result if there is:
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a delay in fixation
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Formaldehyde solutions for routine use are most commonly buffered by:
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monobasic and dibasic phosphates
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How much 95% alcohol must be used to prepare 1000mL of 70% alcohol?
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737mL
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To prepare 500mL of .55% solution, how many grams of potassium metabisulfite would be required?
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2.75
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The symbol posted in an area would indicate which of the following hazards?
a) electrical b) radiation c) biohazard d) explosive |
c) biohazard
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The "high dry" objective usually has a magnification of:
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45x
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Tissue can be fixed in the microwave oven because of:
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rapid molecular movement
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A solution contains 20g of solute dissolved in .5L of water. What is the percentage of this solution?
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4%
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The amount of time a specimen needs to remain in the decalcifying solution is NOT influenced by the:
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processing schedule
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Epon functions as a:
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infiltating medium
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The process of saturating tissue with the medium that will be used for embedding is called:
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infiltration
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The alcohols on the automated tissue processor should be changed on a regular basis because:
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the alcohols can absorb moisture and become dilute
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A clearing agent must be miscible with:
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dehydrants and infiltrating media
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Hemosiderin-laden macrophages present in the alveolar spaces of the lung can be distinguished from other pigmented macrophages by staining with:
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prussian blue
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In staining methods for demonstrating reticulin, potassium permanganate, phosphomolybdic acid, and periodic acid function as:
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oxidizers
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A combination method for demonstrating fat and myelin in frozen sections of brain is the Luxol fast blue combined with:
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oil red O
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Which of the following staining procedures is most suitable for demonstrating general tissue morphology?
a) PAS b) Verhoeff-van Gieson c) H&E d) Wilder reaction |
c) H&E
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Microscopic review of a section stained with the Warthin-Starry technique show the spirochetes stained yellow. The most likely cause is:
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underdeveloping
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An example of an exogenous pigment is:
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carbon
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The bacterium Legionella pneumophila can be demonstrated using which of the following staining methods?
a) Schleifstein b) Pinkerton c) May-Grunwald d) Steiner |
d) Steiner
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Melanin is normally found in the:
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skin
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Carmine and aluminum chloride combine to form Mayer:
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mucicarmine
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A staining method considered to be most sensitive and specific for copper is the:
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rhodanine
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A technique for demonstrating calcium, wherein sections immersed in silver nitrate solution are exposed to bright light, is the:
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von Kossa
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An organism that is resistant to decolorization after staining with a basic aniline dye followed bygan iodine mordant, is referred to as:
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gram positive
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In the Hall method, Fouchet reagent is used to demonstrate:
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bile
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The property of "acid fastness" appears to be related to the walls of organisms which contain:
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lipids
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The term "argentaffin" literally denotes a reaction wherein cells have the ability to reduce a salt of:
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silver
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The process by which the mineral content of tissues can be studied following removal of organic tissue components is called:
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microincineration
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The addition to thymol crystals to staining solutions used for demonstrating microorganisms serves to:
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c) inhibit mold growth
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Which of the following methods use a methenamine solution?
a) Foot-Bielschowsky and Bodian b) Wilder and Snook c) Jones and Gomori d) Gridley and Laidlaw |
c) Jones and Gomori
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The phosphotungstic acid-hematoxylin (PTAH) stain is useful for demonstrating:
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muscle striations
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Pigment that is present on the surface of cells but not within them is probably:
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artifact
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In silver impregnation techniques, the use of improperly cleaned glassware results in:
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nonselective silver precipitation
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A delicate three-dimensional connective tissue meshwork that forms the framework of organs, such as the spleen and lymph nodes, is made of:
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b) reticular fibers
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