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39 Cards in this Set
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- Back
- 3rd side (hint)
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Histopathology |
Anatomic pathology producing a quality tissue section |
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Marie Francois Xavier Bichat |
Father of Histology |
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Johaness Peter Muller |
Father of histopathology and cellular pathology |
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Ferdinand Blum |
Propoaed the use of Formaldehyde as a fixative |
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Numbering |
Assigning number of specimen in log book |
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Gross Examination |
Slicing/Placement of specimen in tissue cassettes |
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Fixation |
Preserving tissue specimen (life-like manner) 10% formalin |
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Decalcification |
Removal of calcium from SOME tissue or organs Nitric acid |
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Dehydration |
Removing water from specimen using Ethyl alcohol |
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Clearing |
Remove excess alcohol and makes tissue transparent Xylene |
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Inflitration |
Filling up tissue spaces/cavities with Melted paraffin wax |
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Embedding |
Placing the infiltrated tissue inside a mold |
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Trimming |
Removing excess paraffin wax from the block |
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Sectioning |
Cutting of tissue block into thin slices called ribbons/sections using a microtome |
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Microtome |
Used for cutting blocks into sections |
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Rotary microtome |
Most common type of microtome |
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Staining |
Employs the use of hematoxylin and eosin dyes to differentiate cells/cell parts |
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Mounting |
Putting the cover slip on the stained tissues using a mounting medium |
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Labeling |
Specimen number is indicated on the glass slide |
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Hematoxylin and eosin dyes |
Dyes used for staining in routine examination |
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Fine Needle Aspiration Biopsy |
Investigating superficial masses or lumps to detect any pathologic condition like malignancy Hollow needle in mass for collection |
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Papanicolaou Smear |
Screening for cervical cancer, STD's such as trichomoniasis, candidiasis, and human papillomavirus (HPV) |
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Cervical cancer, STD's: Trichomoniasis, Candidiasis, and human Papillomavirus (HPV) |
Pap smear is used to detect/screen: |
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Cell Block |
Paraffin-embedded specimen prepared from dried mucus, sputum, and debris found in body fluids |
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Plasma and thrombin or melted agar |
Used to solidify residue in cell block |
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Cytospin |
Concentrate cells on a slide in a uniform monolayer using high speed centrifuge. Like cell block, but skip plasma/agar |
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Frozen Section |
Performed when an immediate or rapid microscopic analysis of specimen is needed. |
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Cryostat |
Microtome used in Frozen section |
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Breast Panel |
Biomarkers important in the genetic testing for breast cancer |
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Estrogen receptor, progesterone receptor, Her2-neu, P-53, DNA ploidy analysis |
Different tests in Breast Panel (5) |
EPHPD |
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Histochemistry |
Uses special stains to determine the chem. compounds and their distribution within and in-between the cells of the body |
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Immunohistochemical Staining |
Detects antigens in the cells of tissue sections by using antibodies (BROWN) diaminobenzydene |
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Diaminobenzydene |
Brown precipitate in staining is: |
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Post-mortem Examination/Autopsy |
Examination of dead body to determine the cause of death, manner of death, and to evaluate any disease or injury that may have been present |
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Nitric acid |
Routine decalcifying agent |
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10% formalin |
Routine fixative |
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Ethyl alcohol |
Used for routine dehydration |
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Xylene |
Routine clearing agent |
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Melted paraffin wax |
Used for infiltration |
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