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37 Cards in this Set
- Front
- Back
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Why must bacteria excrete exoenzymes to break down large molecules into smaller molecules?
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The enzymes degrade the large molecules into smaller molecules so that the small molecules can be transported into the cell.
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What kind of enzyme is able to degrade starch?
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amylase
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What kind of enzyme is able to degrade cellulose?
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cellulase
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What are the resulting end products when starch is degraded?
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simple sugars
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What are the resulting end products when cellulose is degraded?
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simple sugars
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Starch is a
(A) nucleic acid (B) carbohydrate (C) fat (D) protein |
B
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Cellulose is a
(A) nucleic acid (B) carbohydrate (C) fat (D) protein |
B
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What kind of enzyme is able to degrade a polypeptide (large protein)?
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protease
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What are the resulting end products when proteins are degraded?
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amino acids
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Casein is a
(A) nucleic acid (B) carbohydrate (C) fat (D) protein |
protein
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Gelatin is a
(A) nucleic acid (B) carbohydrate (C) fat (D) protein |
protein
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What kind of enzyme is able to degrade fat?
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lipase
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What are the resulting end products when fats are degraded? 2
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fatty acid
glycerol |
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What are the end products of the hydrolysis of tryptophan? 2
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pyruvate
indole |
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What are the end products of the hydrolysis of urea?
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ammonia
carbon dioxide |
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The starch molecule is composed of which two types of molecules? 2
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amylose
amylopectin |
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What is the indicator (test reagent) that is used to "develop" a starch agar plate?
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Gram iodine
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What does a positive test result for starch hydrolysis look like?
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clear zone surrounding bacterial growth
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The degradation of proteins into peptides and amino acids is known as…
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proteolysis
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What type of medium is used to test for casein hydrolysis?
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skim milk agar
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What does a positive test for casein hydrolysis look like?
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clear zone surrounding bacterial growth
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What type of medium is used to test for fat hydrolysis?
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spirit blue agar
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What does a positive test result for fat hydrolysis look like?
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formation of dark blue precipitate
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A positive test result for fat hydrolysis may result from what change to the pH of the medium?
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lowering pH
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What type of medium is used to test for tryptophan hydrolysis?
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tryptone broth
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The enzyme responsible for hydrolysis of tryptophan is called?
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tryptophanase
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What does a positive test result for tryptophan hydrolysis look like?
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red ring or band at the top of the tube
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What is the indicator (test reagent) that is used to test for hydrolysis of tryptophan?
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Kovac's reagent
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The enzyme responsible for the hydrolysis of urea is called?
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urease
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What does a positive test result for urea hydrolysis look like?
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bright pink
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What indicator is used in the urea medium?
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phenol red
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Does urea hydrolysis result in an increase or decrease in pH?
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increase
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What is the name of the enzyme that helps to break down phenylalanine into phenylpyruvic acid?
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phenylalanine
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What reagent is needed to complete the test for presence of phenylpyruvic acid?
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ferric chloride
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What does a positive test for presence of phenylpyruvic acid look like?
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green
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What medium is used to test for phenylalanine degradation?
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phenylalanine agar
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What enzyme causes phenylalanine degradation?
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phenylalanine deamininase
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