Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
60 Cards in this Set
- Front
- Back
Give the laboratory diagnosis of viral infection |
1-5 |
|
How do you collect in specimen collection |
Aseptically collection |
|
Delayed specimen temp |
1-6°C |
|
Delayed temp hour |
Less than 24 hrs |
|
Long storage temp |
-70°C |
|
Long temp sotrage: |
Freezer |
|
-70°C in long temp called |
Cryoprecipitate |
|
Refrain storage temp |
-20°C |
|
Wht refrain the temp bc |
It may cause formation of crytals that will inactivaye the virus |
|
swab Specimen collected by |
Dacron Rayon Cotton Polyester |
|
Swab specimen examples! |
TENS |
|
Type of swab that is not allowed to to be used becayse it may cause damage or inactivate the virus |
Calcium alginate |
|
Specimen does not need transport mediym |
Blood Csf Urine Bonemarrow Pericardial Pleural Synoviql |
|
Vtm means |
Viral Transport medium |
|
If your specimen doesn't have vtm: |
Centri - 3000rpm 15-30 mins |
|
Specimen that needs VTM |
-specimen collected by swab - Respiratory specimen -Tissue Biopsy |
|
Examples of respiratory viruses |
IAFCPR |
|
In nasopharyngeal must use _____ swab insert _________ with ______ |
Flexible 2-3cm NSS |
|
Examples of respiratory specimen |
Throat Nasopharyngeal |
|
VTM contains? |
Trypticasw Soy Broth, NSS |
|
VTM contain TSB and NSS solution is |
Avoiding drying of the specimen |
|
Vtm also contain |
Gelatin, serum, albumin protein |
|
Vtm contains g.a.s.p solution: |
To protectthe less viable viruses |
|
Vtm has ---- for inhibition of any growth |
Antibiotic and Antifungal |
|
Vtm has antibiotic and Adntifungal solution |
For inhibition of any bacterial and fungal growth |
|
Methods of Viral isolation |
Cell culture Animal inoculation Embryonated egg inoculation |
|
In vitro is |
Cell culture |
|
In vivo is |
animal inoculation and Embryonated egg inoculation |
|
in cell culture if you dispense THE cell in tissue by----- using ----- |
Grind or mince Mortar and Pestle |
|
It is a Gold standard and presumptive test at the same time |
Cell culture |
|
Enumerate the categories of preparation of cell culture |
Primary cell culture Finite cell culture Continuous cell culture |
|
Deruved from an animal cell |
Primary cell culture |
|
In primary cell culture it has |
Rk and pMk |
|
A tissue is treated with enzyme to separate the cells what enzyme |
Trypsin |
|
Grow in monolayer across the glass |
Normal cells and Primary cells |
|
Do not grow in monolayer across the glass or plastic container |
Transformed cells and Continuous cell culture |
|
Cells are capable of |
cell division and splitting and fasting |
|
Transfer cell container to another to treat again with trypsin |
Finite cell culture |
|
50 generation |
Finte cell culture |
|
ex of finitecc |
HUman diploid fibroblasts Human neonatal lung |
|
Heteroploid |
Normal and abnormal tissue |
|
Ex of continuous cell culture |
A549 HEP2 |
|
what is a549 and HEP2 |
Human lung carcinoma cell line Human Neonatal lung |
|
Observation in cytophatic effect |
Change in size Change in shape Syncytia |
|
What is syncytia |
fusion of 2 or more cell |
|
Non culturable |
Hepatitis B virus |
|
culturable in tissue cells |
M. leprae |
|
HsV |
- +++ +++ +++ +++ |
|
CMV |
- +++ - - - |
|
VZV |
- +++ - - +/- |
|
Enterovirus /mc |
+ + ++ - + |
|
RSV/ pauline |
-/+ + +++ - ++ |
|
Influenza and Parainfluenza virus/ mama at papa |
+++ +/- - - - |
|
Adenovirus /lola at lolo |
+ ++ +++ ++ ++ |
|
Large rounded cells |
Hsv cmv |
|
Foci or rounded cells possible of syncytia |
VZV |
|
Refractile round cell in clusters |
Enterovirus |
|
Syncytia |
rsv |
|
Variiable no cp3 |
iP |
|
Large rounded cell in clusters |
adenovirus |