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28 Cards in this Set
- Front
- Back
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Solution Concentration |
Weight/Volume: weight out solute, add to volumetric flask and then fill to 100mL
Weight/Weight: Weight out solute and add to a beaker, and then add solvent until it reaches 100g
Volume/Volume: Add amount of solute desired in mL to a volumetric flask, then fill to 100mL with solvent. |
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Benedict's test |
Reacts with reducing sugars when boiled, converting Cu-II to Cu-I (Blue to red)
Tablet form is called Clinitest |
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Glucose Oxidase |
Converts glucose to H2O2 and the peroxidase converts it to a dye measured at 500nm
Most specific for Beat-D-Glucose |
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Glucose oxidase samples |
Blood glucose decreases by 7% per hour of not properly stored.
Stable for 24 hours if refrigerated and removed from the clot. |
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Glucose Oxidase Interfeirnces |
Grossly lipemic samples |
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Glucose Oxidase Normal ranges |
70-105mg/dL |
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Glucose Hexokinase |
Glucose is phosphorylated in the presence of magnesium and ATP
Glucose 6 phosphate is then oxidized in the presence of NAD, producing NADH. This creates a measurable increase in absorbance |
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Glucose Hexokinase samples |
Stabled for 4 days at 4c if removed from the clot.
severly Lipmeic or hemolized samples should not be used. |
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Glucose Hexokinase normal range |
65-110mg/dL
Range: 0-600mg/dL |
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DNSA |
Detects reducing sugars. Changes from yellow to red when boiled with them.
Not as popular as Benedicts |
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A1C normal ranges |
4-6%
Can be up to 15-20% in diabtics
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A1C test purpose |
Monitores Glucose levels over the past 4-8 weeks |
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A1C storage |
a week if refrigerated |
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A1C test |
Goat-Antimouse polyclonal Ab agglutinates with Ag on a latex bead after binding to A1C on RBCs |
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A1C interferiences |
Opiate addiction, Lead poisoning, alcoholism, or large asprin doses. |
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Diabetes Diagonoses |
Glucose greater than or equal to 200mg/dL regardless of last meal time
or
a fasting glucose of 126mg/dL or greater |
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Hypoglyciemia |
Blood glucose less than 150mg/dL |
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Lactose intolerance causes |
Natural reduction of Lactase after infancy
Damage to the intestinal lining |
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Lactose Intolerance testing |
Mucosal biopsy to test for lactase
Breath hydrogen test: 2-5uL/L is normla, 30-100uL/L is lacotse intolerance |
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Biuret test |
Minimum of two pepetides bond for reaction
Peptide bonds react with Cu2+ in alkaline solutions resulting in a blue product that reads out at 540nm
measures total protien |
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Total protein specimens |
Lipemia and gross hemolysis causes false results
Stable for 1 week at room temprature or one month if refrigerated |
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Total protein ranges |
healthy 6.3-8.3g/dL in mobile adults
6.0-7.8g/dL in imobile adults
range: 1-15g/dL |
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Albumin Sample |
Avoid hemolysed samples
Stores for 1 week at room temp or 1 month if refrigertated. |
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Albumin dyes |
BCG or BCP (purple or green)
Ampacillin and penacillin interfier with BCG |
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Albumin ranges |
3.2-5.2g/dL |
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See slides 30-37 |
XXX |
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Electrophorieses trouble shooting |
Distorted bands: Bent applicators, air bubbles during application, to much sample, to much drying during electrophoreises
Unusual bands: Artifacts from hemolyzed samples, band at starting point is likely fibrinogin |
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Immunofixation electrophoresis |
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