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10 Cards in this Set
- Front
- Back
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Define "aeseptic technique"
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Techniques that aim to prevent contamination.
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What instrument is used to inoculate each of these?
1. broth culture 2. deep 3. petri plate medium 4. slant |
1. loop
2. needle 3. loop 4. loop |
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When streaking for isolation: streak #1 is followed by flaming the loop. Do you place more bacteria on the slide for streak #2?
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No. Only place loop in bacterial broth once.
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Name the organism used in the lab to inoculate the broth, slant, and deep.
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Serratia marcescens.
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What is the difference in procedure when preparing a bacterial smear on a slide from a broth vs. a solid culture?
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Broth- use no water.
Solid- place water on slide first. |
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What is the purpose of heat-fixing the bacterial smear?
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It denatures the bacteria so it sticks better to the slide.
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Is the dye Methylene Blue acidic or basic?
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Basic (positively charged).
The bacteria is negatively charged so a basic dye will attract better to it. |
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Why is the negative staining procedure better than the simple stain when observing morphologies?
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The negative stain does not stain the bacteria, but only the surrounding area. Dyeing the bacteria could alter the morphology.
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What dye was used in the simple stain?
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Methylene Blue.
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What dye was used in the negative stain?
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Nigrosen.
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