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10 Cards in this Set
- Front
- Back
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In water analyses, name 2 choliforms and state the difference
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E.coli makes acid & green sheen
Enterobacter can also ferment lactose to acid & gas, but not enuf to make blue/pink colonies. Green sheen also. |
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What method is preferred for confirmed water test? What does ENDO do & what color?
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EMB/ENDO.
ENDO pulls fuschin dye into colony and becomes rose red |
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AMA? 3 types?
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AMA= Antimicrobial Agents
Antiseptic, Disinfectant, Antibiotic |
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Antiseptic? Disinfectant?
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Antiseptic: Mild CHM, de-germing. Topical. Ex: Vespene
Disinfectant= strong CHM, not on body, but on surfaces. Ex: Phenol |
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Antibiotic?
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Internal CHMs. Has inactive specific structures & functions. Interferes with. ribosomes. Limited against Fungi bcs also Euk. with 80 ribosomes.
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MIC?
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Minimum Inhibitory Concentration of an AMA
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Tube Dilution test?
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Bacterium into broth tubes with serial dilutions of antimicrobial agents. Incubate. Lowest conc. of agent that shows no growth is MIC of antimic
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Disk Diffuesion method?
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Called Kirby-Bauer test. Agar stamped with antimics. Circular zone around disk shows inhibition. Foogy=resistant. Clear, sharp=best
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Name 2 antimicrobials from Lab16. Which was antiseptic?
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5% phenol & 5% formaldehyde are antimicrobials. 5% iodine is antiseptic. All 3 are antibiotics
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Which is halo-tolerant, but not at 25%?
Gm from water usually? What are enterics? 2 choliforms that inhabit colon? |
Staph. Gm(-). Enterics are in intestine and Glucose + , oxidase -
E.coli & Enterobacter Aerogenes (non-fecal). |
