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43 Cards in this Set
- Front
- Back
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What type of microscope would you use to show outline, some internal structures, and some external structures? |
Compound Light Microscope |
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What is the highest amount of magnification you can get from a compound light microscope? |
1000x |
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What provides initial magnification on a compound light microscope? |
objective lens |
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How much magnification do you get when using a scanning lens on a compound light microscope? |
4x |
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How much magnification do you get when using a low power lens on a compound light microscope? |
10x |
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How much magnification do you get when using a high power lens on a compound light microscope? |
40x |
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How much magnification do you get when using the oil immersion lens on a compound light microscope? |
100x |
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Which lens re magnifies image formed by the objective lens? |
ocular lens |
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How much magnification does the ocular lens provide on a compound light microscope? |
10x |
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What is the total magnification on a compound light microscope? |
objective lens x ocular lens |
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What is the ability of the lenses to discriminate 2 points tat are at a specific distance? |
Resolving power/resolution |
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What happens to magnification as resolving power gets smaller? |
Magnification increases |
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What kind of microscope observes live organisms with a black background? |
Darkfield Microscope |
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What type of microscope observes live organisms with a black background, but can see internal structures, and is clearer than the Darkfield microscope? |
Phase-Contrast Microscope |
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What microscope uses an UV light and is useful for diagnostic microbiology? |
Fluorescence microscope |
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What type of microscope takes 2-3 dimensional images with a laser, and can analyze an entire organism? Also uses a UV light |
Confocal microscope |
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How much better resolution does the Confocal microscope have than the fluorescence microscope? |
40% better resolution |
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What type of microscope uses electrons instead of light? |
Electron microscope |
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What type of lenses does an electron microscope use instead of objective lenses? |
electromagnetic lenses |
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What are the two types of electron microscopes? |
Transmission and Scanning |
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Which electron microscope would you use to see detailed internal structures? |
Transmission EM |
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What is the magnification range of a Transmission EM? |
10,000-100,000x |
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Which electron microscope would you use to get 3D views? |
Scanning EM |
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What is the magnification range of a Scanning EM? |
1000-10,000x |
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What is the resolving power of a Transmission EM? |
2.5 nm |
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What is the resolving power of a Scanning EM? |
20nm |
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What are the 2 types of dyes? |
Basic and acidic |
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Which dye would you used for positively charged samples? |
Basic |
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What are the 3 types of basic dyes? |
crystal violet, methylene blue, malachite green |
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What do you stain when using basic dyes? |
the organism |
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Which dye would you use if you sample was negatively charged? |
Acidic |
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What are the 3 types of acidic dyes? |
eosin, acid fuchisin, nigrosin |
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What do you stain when using acidic dyes? |
the background, not the organism |
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What does simple staining determine? |
If bacteria is in sample, shape, and arrangement of bacteria |
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Which type of staining method only uses 1 type of dye? |
simple staining |
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Which type of staining method uses more than 1 type of dye? |
Differential staining |
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What are the 4 steps of Gram staining? |
1) dye 2) mordant (iodine) 3) decolorization (ethyl alcohol) 4) counterstain |
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If a sample is purple, is it gram positive or gram negative? |
gram positive |
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If a sample is pink, is it gram positive or gram negative? |
gram negative |
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Why type of staining would you use if there is an atypical cell wall (such as with microbacterium with a waxy cell wall) |
Acid-fast staining |
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Which staining technique is used to observe bacterial capsule? |
Negative stain |
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Why do we need an endospore stain? |
Gram stain cannot go through the walls of an endospore |
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Why do we need a flagella stain? |
Flagella are too small to see with regular stain |
