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6 Cards in this Set

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  • Back

Most simple stains contain

Synthetic aniline dyes(coal tar derived


Dyes derived from benzene


Usually salts but can be acid or bases


What is the purpose of heat smear?

Kills the cells


Deactivates enzymes that lyse cells


Makes cells stick to slide


In heat fixing what would too much heat do?

Cells damage and not stain properly

Value of simple stain

View cell shape morphology and arrangement

What medium type needs water before smearing

Solids

How to perform smear of liquid medium

Two loopfuls of culture on slide with sterile loop


Spread bacteria in circle


Let it to air dry


Either heat fix or cover smear with 95 percent methanol one minute


Let air dry