Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
13 Cards in this Set
- Front
- Back
- 3rd side (hint)
|
What is dna replicated by? |
DNA polymerase |
|
|
|
What does DNA polymerase need to start replication |
Primers |
Base of makeup |
|
|
What happens when dna is unwound? |
Hydrogen bonds are Brocken |
|
|
|
How is the leading strand replicated |
Continuously |
Forever |
|
|
How is the lagging strand replicated |
Fragments |
Small |
|
|
What are the fragments joined by? |
Ligase |
|
|
|
In PCR what temperature is dna heated at and why is it heated |
92-98 to separate strands |
|
|
|
It is then cooled what temperature is it cooled at and why? |
50-65 to allow primers to bind to target sequence |
|
|
|
It is reheated at what temperature and why? |
70-80 for heat tolerant DNA polymerase to replicate the region of DNA |
|
|
|
What is a good use of PCR |
To help solve crimes, settle paternity suits and diagnose genetic disorders |
|
|
|
What are the three types of RNA involved in translation and transcription? |
MRNA (messenger) RRNA (ribosomal) TRNA (transfer) |
M R T |
|
|
What is removed during RNA splicing? |
Introns |
|
|
|
What is a phenotype determined by? |
Proteins and environmental factors |
|
