Buffer solution

better blank to use in this experiment. The better blank solution would have been the one with active enzyme because it would cause the spectrophotometer to measure the absorbance of every component in the blank except the substrate, creating a 100% transmittance. This allow the spectrophotometer to read everything that was not in the blank when the actual sample is inserted in because it would “blank” out all the components in the “blank” solution and only read the “uncommon” component. With…

located in the mitochondrial membrane and aids in cell respirations and enzyme generation. In order to conduct this experiment, students cut a liver and through a process created six fractions. Each of the six fractions was moved to a cuvette with solutions containing different volumes and recoding the absorbance every five seconds for four minutes. Once the absorbance was obtained for each, we determined that cuvette E (0.0012) had the least activity per milliliter while cuvette B had the most…

(II) in simulated wastewater by emulsion liquid membrane technology (ELM). It has been transported of nickel ion (II) and Cadmium (II) from aqueous solution. Which contains a single compound system and A complex system through the emulsion liquid membrane emphasis was placed on it. It was the use of the effect of the concentration of citrate buffer solution and mobile carrier and stripping phase. This parameter represents a very important factor to affect Ni(II) and Cd(II) in flux through in…

Turbidity: - Turbidity is a degree of water clarity. Turbidity is defined as an “expression of the optical property that causes light to be scattered and absorbed rather than transmitted in straight lines through the sample”. Nephelometers are used to measure the intensity of light scattered by contaminants present in water such as suspended and colloidal solids like clay, silt and microscopic organisms. Turbidimeters using the principle of nephelometer compare the light scattered due to…

In the second part of the experiment, KHT was added to solutions of different potassium ion concentrations. Similar to the first part, the concentration of HC4H4O6- was calculated through titrations of 0.05 M KCl and 0.1 M KCl solutions with known NaOH concentrations. The total concentration of K+ for the solutions with the common ion is equal to the K+ concentrations from the KCl solutions and the K+ concentration from the KHT itself, which is equal to the concentration…

observe osmosis and what happens when the membrane is presented with different solutions. Since we did not know the concentration of the egg, we had to assume that it equaled the concentration of the membrane. We then had the ability to determine the concentration based off the assumption and the rate of osmosis. We used 5 different sucrose solutions: 0%, 10%, 20%, 30%, and 40%. One group had an ‘Unknown’ sucrose solution, this would give us the opportunity to use all of our data collected…

In this study, the changes in the rheological curves of polyvinyl chloride (PVC) plastisols with increasing storage time and the factors affecting the changes were studied,. The results show that with increasing storage time, all the "viscosity–temperature" and "viscosity–time" rheological curves of PVC plastisols exhibit non-normal distribution change trends, i.e., the viscosity first decreases, and then changes from slow increasing to rapid increasing, forming a shoulder peak, reaches to the…

4.5.3 Texture Acceptability of Mushroom Soup In term of texture, the acceptance of particle in soups was determined (Table 4.3). Generally, soup prepared from freeze dried powders had higher scores for acceptability of 4.47 ± 1.10 than oven dried and vacuum dried powder that varies between 3.80± 1.06 and 4.47± 1.43. However, there was no significant difference (p> 0.05) among the soups prepared with fresh mushroom and powders in the respect of texture. The lower texture acceptability of oven…

concentrations of water to low concentrations of water through a selectively permeable membrane. Osmosis is a way for water molecules to create equilibrium between a cell and its environment. For example, if the cell is a hypotonic solution and its environment is a hypertonic solution, then water will naturally diffuse to its environment to create equilibrium. The guiding question for this experiment was, “How does alcohol concentration affect the water content in cells?” It is related to…

compartment (with a higher concentration). In hypotonic solution, the concertation of a substance is larger within the cell; the cell gains water to dilute this concertation . in hypertonic solution, the concentration of substance is larger in solution than the cell. The cell will…