5For numerous reasons, Type II restriction enzymes are found to be the most valuable in the laboratory. Several of the reasons making these enzymes the most suitable is that no ATP is required when cutting DNA, the cuts made are steady and dependable as they remain on or very close to the recognition sites, and they all merely have restriction activity and no modification activity.4 Within this experiment, three Type II restriction enzymes were used and they include Bgl I, Hind III, and PVU II. Hind III, which is created by Haemophilus influenza, reacts at a temperature of 37°C and cleaves DNA at the particular sequence 5’AAGCTT 3’ when Mg2+ is present.6 PVU II, which is produced from Proteus vulgaris, both recognizes then cuts the DNA sequence, which is double stranded, at 5’ CAGCTG 3’. In addition, PVU cleaves after G-3. Bgl I, which is created from Baccilus globigii, reacts at a temperature of 37°C and cuts the DNA at the specific sequence 5’ AGATCT
5For numerous reasons, Type II restriction enzymes are found to be the most valuable in the laboratory. Several of the reasons making these enzymes the most suitable is that no ATP is required when cutting DNA, the cuts made are steady and dependable as they remain on or very close to the recognition sites, and they all merely have restriction activity and no modification activity.4 Within this experiment, three Type II restriction enzymes were used and they include Bgl I, Hind III, and PVU II. Hind III, which is created by Haemophilus influenza, reacts at a temperature of 37°C and cleaves DNA at the particular sequence 5’AAGCTT 3’ when Mg2+ is present.6 PVU II, which is produced from Proteus vulgaris, both recognizes then cuts the DNA sequence, which is double stranded, at 5’ CAGCTG 3’. In addition, PVU cleaves after G-3. Bgl I, which is created from Baccilus globigii, reacts at a temperature of 37°C and cuts the DNA at the specific sequence 5’ AGATCT