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44 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Platelet |
Small, anucleate, colorless, moderate reftractile bodies |
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Add more squares until 100 platelets are counted |
<100 PLT means? |
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Azure granules |
PLT count appear as _______ with scanty light blue cytoplasm when stained |
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Aggregate and Adhere |
PLT count is characterized by the tendency to ____________ foreign surfaces |
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EDTA |
What is the anticoagulant used for PLT count? |
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True |
PLTs disintegrates easily when exposed to air. T or F |
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1. PLT count simply platelet aggregation and clotting 2. Imperfect sources of blood 3. Procedure during blood extraction 4. Blood in EDTA kept at 20 deg C is satisfactory only within 5 hours after collection 5. Some diluted specimen is only stable for the first 30 minutes |
What are the sources of error? |
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Rees-Ecker Guy and Leeke’s Brecker-Cronkite Unopette Van-Allens |
What are the methods for Direct PLT count? |
BUGRV |
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Dameshek Fonio’s Olef Smear estimation |
What are the methods for indirect PLT count? |
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Brilliant Cresyl Blue (stain) 0.1 g 40% formal 0.2 mL Sodium Citrate 100 mL |
What are the fluids of the Rees-Ecker? |
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0.5 mark |
When using Rees-Ecker, draw blood up to ______ mark of the RBC pipette |
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1:200 dilution |
What is the dilution of Rees-Ecker? |
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Tertiary 25 squares of the central secondary square with 1mm2 area |
Rees-Ecker is counted at? |
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Light blue |
What is the color of the PLT in Rees-Ecker method? |
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Ammonium Oxalate Method |
Draws blood up to 1.0 mark of the RBC and dilutes blood with its fluid up to 101 mark |
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1:100 dilution |
What is the dilution of Ammonium Oxalate? |
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Count all the platelets in all tertiary 25 squares of the central secondary square with 1mm2 area. |
Ammonium oxalate method is counted at? |
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Colorless, refractive bodies |
Platelets of Ammonium oxalate method shows |
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1% Ammonium Oxalate |
Brecker-Cronkite method uses what fluid? |
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1.0 mark |
In Brecker-cronkite method, draw blood up ____ mark of the RBC pipette |
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10 R squares of each counting chamber, total of 20 R squares |
Brecker-cronkite method counts in? |
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Allowable platelet difference of each counting chamber> |
+/- 10 PLT means? |
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Repeat mixing and charging to verify the test |
>10 PLT means? |
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Add more squares until 100 platelets are recorded |
<100 PLT means? |
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In 50 squares, repeat procedure with 1:20/1:10 dilution with WBC pipet |
>50 PLT means? |
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Crystal violet (stain) 40% Formalin 0.2 mL Sodium Citrate 100 mL |
What is the diluting fluid of Guy and Leake’s Method? |
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Modified Rees-Ecker Method |
What is the other term for Guy and Leake’s? |
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Isotonic |
What is the diluent (hyper, hypo, iso) of Guy and Leake’s Method? |
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2000 |
What is the correction factor of Guy and Leake’s method? |
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Lilac refractive bodies |
Appearance of PLT when using Guy and Leake’s method |
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Unopette Method |
A system of prefilled blood vials containing solutions that will preserve certain cell types while lysing the others |
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Unopette Method |
Utilizes a pre-measured volume of diluent in a chamber into which a specified amount of blood is drawn |
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Self-filling capillary pipette |
The unopette test system utilizes? |
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Unopette Method |
Consists of a straight, thin-wall, uniform-bore plastic capillary tube fitted into a plastic holder |
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Plastic reservoir |
Unopette method has a _______ containing volume of reagent for diluting |
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Type of capillary used |
Unopette method’s dilution is determined by ___________ since each type have different volumes |
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Fonio’s Method |
Uses 14% Magnesium sulfate and Wright/Giemsa Stain |
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1:3 |
What is the ratio of diluting fluid in Fonio’s method? |
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Dameshek Method |
This method uses Rees-Ecker Fluid |
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14% magnesium fluid Giemsa or Wright’s stain |
Fluid and Stain used for Olef Method is? |
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1:5 |
What is he ratio of diluting fluid for Olef Method? |
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Thrombocytopenia |
<1 PLT/OIO field |
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Adequate |
5-20 PLT/OIO field |
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Thrombocytosis |
>25 PLT/OIO field |
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