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36 Cards in this Set
- Front
- Back
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based on charge |
ion exchange chromatography |
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based on molecukar suze |
gel filtration chromatography |
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selective binding to a specific molecule |
affinity chromatography |
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based on charge and molecular size |
gel electrophoresis |
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bas3d on isoelectric point of the protein |
chemical precipitation |
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generally used support medium is cellulose or thin gels made up of either polyacrylamide or agarose |
Gel Electrophoresis |
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generally used suppory medium of gel electrophoresis |
Cellulose |
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used as support medium for low molecular weight biochemicals such as amino acids and carbohydrates |
polyacrylamide |
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used as support medium for large molecules like protein and nucleic acid |
agarose |
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done in serum samples to resolve albumin and the globulins into two or more fractions that can then be measured for protein content |
chemical precipitations |
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what can be added in a chemical precipitation in order to precipitate globulins in the solution |
sodium sulfate sodium sulfite ammonium sulfate methanol |
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in chemical precipitation what substance in a serum does precipitate |
Globulin |
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in chemical precipitation what substance tends to left in the solution |
Albumin |
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has been used extensively because it can accentuates abnormalities in serum protein decompositiob |
A/G Ratio |
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preparative procedures for the isolation of a single minor protein constituent usually begins with--## |
Precipitation Step |
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column seperations |
Ion Exchange Chromatography Gel Filtration Chromatography Affinity Chromatography |
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involves protein isolation on the basis of their molecular size and charge |
Column Separation |
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The order of protein elution is by molecular weight or size from largest first to smallest last |
Gel Filtration Chromatography |
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it requires that the medium be inert and do not interact chemically or by charge in the proteins |
gel filtration chromatography |
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it is not a method to do for high resolution |
Gel filtration chromatography |
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takes advantage on the charge on proteins |
Ion Exchange Chromatography |
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begins at a basic pH,mobility is retarded according to net negative charge |
Anion exchange |
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begins at an acid pH with the proteins having positive charge and adhering to negative charge column matrix |
cation exchange |
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Antigens and antibody |
affinity chromatography |
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clevage poin of trypsin |
L,R (C) |
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cleavage point of submaxillarus protease |
R (C) |
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cleavage point of chymotrypsin |
Y,W,F (C) |
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Cleavage if staphylococcus aureus V8 protease |
D,E (C) |
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Asp-N-Protease |
D,E (N) |
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pepsin |
F,W,Y (N) |
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endoproteinase |
K (C) |
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Cyanogen bromide |
M (C) |
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proteins found in blood plasma |
plasma proteins |
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maintenance if water distribution between cells and tissue, interstitial compartments and the vascular system of the body |
osmotic force |
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participation as buffers to maintain pH |
Acid-Base Balance |
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reagent in edman technique |
PITC |
