coli culture by inducing gene expression. There are two ways to typically induce gene expression in E. coli. Lactose is a common way to activate promoters in E. coli, and in this lab a chemical IPTG was used. The promoter in the pFG gene can be activated by IPTG and the gene will be inactive if IPTG is absent. Another common promoter is arabinose. When this sugar is present, the pGlo promoter will be turned on, and when it is absent, the promoter will be turned off. Therefore, based on which plate displayed the fluorescent phenotype, the plasmid can be
coli culture by inducing gene expression. There are two ways to typically induce gene expression in E. coli. Lactose is a common way to activate promoters in E. coli, and in this lab a chemical IPTG was used. The promoter in the pFG gene can be activated by IPTG and the gene will be inactive if IPTG is absent. Another common promoter is arabinose. When this sugar is present, the pGlo promoter will be turned on, and when it is absent, the promoter will be turned off. Therefore, based on which plate displayed the fluorescent phenotype, the plasmid can be