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12 Cards in this Set
- Front
- Back
What a protective coating around some pathogenic bacteria that protects them from the phagocytic activity of the host?
What is the stain used to ID this coating? |
Capsule
Capsule staining |
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Some gram positive (ex: Bacillus) bacteria can live as an inactive cell type resistant to harsh environmental conditions.
What is this cell type called? How is it identified? |
Spore
Endospore stain |
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What are organisms called that are able to resist decolorization with acid-alcohol?
Name a specific example |
Acid-fast
Ex: mycobacterium tuberculosis |
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What is a specific method of capsule staining? What color will the capsules be?
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Anthony's method
A clear halo around purple bacterium and background |
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What is a specific endospore staining method? What color will the spores be against the vegetative cells?
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Malachite Green Method
Green against pink |
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What is a specific method of an acid fast stain?
The non-acid fast organism will appear BLANK, while the acid-fast organism will appear BLANK |
Ziehl-Neelsen
blue red |
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What are viruses that infect bacteria with great specificity?
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Bacteriophages
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What is called when a bacteriophage's genome is immediately replicated & new phage particles are synthesized in the host cell?
What happens next? |
Lytic cycle
The cell lyses and releases the new bacteriophages |
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What is called when a bacteriophage's DNA becomes integrated into the host's genome (prophage) and is replicated?
What happens next? |
Lysogeny
An environmental cue will trigger the lytic cell and then the host cell will lyse. |
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If a phage is incubated along with its appropriate host on an agar plate, it will produce BLANK
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Plaques
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After incubation of a bacteriophage and its appropriate host, BLANK units can be counted on an agar plate.
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plaque-forming units (PFU)
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Simply describe the pour plate technique
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Agar plate sandwich:
bottom: agar middle: broth mix of phage & bacteria top: agar |