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21 Cards in this Set
- Front
- Back
What percentage of oxygen does air contain?
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20%
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Describe the oxygen requirements of obligate or strict aerobes.
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can grow with or without oxygen OR are indifferent to oxygen
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Describe the oxygen requirements of aerotolerant organisms.
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an grow with or without oxygen OR are indifferent to oxygen
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Name the 2 genus of bacteria that are classified as strict aerobes.
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Bacillus OR Pseudomonas
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Name the 2 genus of bacteria that are classified as strict anaerobes.
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Clostridium OR Bacteroides
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Name one genus of bacteria that are classified as aerotolerant organisms.
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Streptococcus
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What role does oxygen play in the metabolism carried out by strict aerobes?
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final electron acceptor
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Name the 2 enzymes that is not present in a strict anaerobe and describe the function of this enzyme.
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catalase
superoxide dismutase enzyme neutralizes by-products of oxygen (free radicals) |
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When a facultative anaerobe is grown in an atmosphere lacking oxygen, what is one type of metabolism that can occur?
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fermentation
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What happens when a candle is lighted and placed inside a closed candle jar? Explain.
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candle is extinguished (2 pts) because oxygen is consumed by the flame
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What enzyme do aerotolerant organisms lack?
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catalase
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What does “FTM” stand for?
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fluid thioglycollate medium
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What does “TGYA” stand for?
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tryptone glucose yeast extract agar
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In a tube of FTM or TGYA, where would you expect to find growth of strict aerobes?
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only at the top of the medium
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In a tube of FTM or TGYA, where would you expect to find growth of strict anaerobes?
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only at the bottom of the medium
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In a tube of FTM or TGYA, where would you expect to find growth of facultatives?
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throughout the central portion (middle) of the medium
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In a tube of FTM or TGYA, where would you expect to find growth of microaerophiles?
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under the top surface of the medium (not at the top of the medium)
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In FTM, what is the role of sodium thioglycollate?
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to reduce the potential for oxidation and reduction
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Why is FTM typically pink at the top and colorless in the middle and bottom of the tube? Explain.
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The dye in FTM (resazurin) turns pink in the presence of oxygen and there is more oxygen near the top of the medium
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Describe the preparation of a TGYA “shake”.
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TGYA is inoculated in the liquid state and then shaken to mix the organisms throughout the medium
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What is the role of FTM tube?
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medium designed to test the aerotolerance of bacteria.
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