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14 Cards in this Set
- Front
- Back
Who discovered the gram staining procedure?
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Hans Christian Gram.
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After a gram positive bacteria is correctly stained; what color is it?
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Purple.
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After a gram negative bacteria is correctly stained; what color is it?
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Red or Pink.
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What does a gram-positive cell and a gram-negative cell have in common in relation to their cell walls?
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Both gram-positive and gram-negative cells have a layer of peptidoglycan in their cell walls.
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What is different in a gram-positive cell and a gram-negative cell in relation to their cell walls?
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A gram-positive cell has a thick layer of peptidoglycan whereas a gram-negative cell has a thin layer of peptidoglycan surrounded by a lipid-filled outer membrane.
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What is removed during the decolorization step on a gram-negative cell?
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The lipid-membrane and the crystal violet-iodine complex.
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Around how many hours can a culture be used until the results might become inconsistant?
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24 hours.
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What is in the gram staining kit?
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Crystal Violet, iodine, alcohol decolorizer and safranin.
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Should you wait for the liquid content to dry on slide before heat-fixing?
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Yes.
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Should you heat-fix before staining an organism?
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Yes.
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What is the first application you use on a gram staining procedure and how long do you keep it on?
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Crystal violet and for 30 seconds.
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What is the second application you use on a gram staining procedure and how long do you keep it on?
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Gram's iodine and for 1 minute.
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What is the third application of a gram staining procedure and how exactly do you execute it and how long do you keep it on?
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Alcohol of decolorizer and hold at an angle when applying and 5 seconds.
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What is the fourth application of a gram staining procedure and how long do you keep it on?
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Safranin and for 1 minute.
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