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17 Cards in this Set
- Front
- Back
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Is the acid-fast stain medically important? If so, how important in relation to gram staining?
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Yes and in relation to the gram stain, it is second in importance.
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Why is acid-fast staining important?
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Acid-fast staining is important because of the highly pathogenic nature of certain members of the genus: Mycobacterium such as M. leprae and M. tuberculosis.
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Why does the genus "Mycobacterium" not completely destain during a gram stain process?
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Because of a high concentration of mycolic acid in their cell wall which produces an irregular effect on the second example of a gram-positive organism.
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Does the mycolic acid in the genus "Mycobacterium" make it easier for an acid-fast stain to identify?
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Yes.
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What does an acid-fast positive cell look like?
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Red.
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Why do acid-fast positive (also known as acid-fast) cells retain their red color?
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Because the carbolfusin in an acid-fast positive cell is more soluble in the cell wall lipids than in acid-alcohol.
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What do acid-fast negative cells look like?
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Blue.
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Why do acid-fast negative (also known as non-acid-fast) cells appear blue?
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Because acid-fast negative cells lack the lipid components and the carbolfushin is rapidly removed during decolorization which leaves the cell colorless. The acid-fast negative cell appears blue after methylene blue is applied as a counterstain.
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What is used as a mordant for an acid-fast stain and what cell layer does the primary stain (carbolfuchsin) penetrate?
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Heat and the carbolfuchsin penetrates the waxy mycolic acid layer.
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Should you wait for your slide to dry before heatfixing.
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Yes.
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Should you heat-fix your slide before staining?
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Yes (always).
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Should you wait till beaker is steaming before putting slide?
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Yes (always).
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What is the first application of an acid-fast stain and what is the purpose of the application.
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Carbolfuchsin and it is the primary stain.
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While on the beaker, should you allow the Carbolfuchsin to dry? If not, should you apply more?
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No you should not allow the Carbolfuchsin to dry and you should should apply more.
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What is the second application of an acid-fast procedure and what is the purpose of the application and how long should the application be?
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Heat and it is the mordant and 10 minutes.
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What is the third application of an acid-fast procedure and how much should you put and what is the purpose of the application and how long should the application be?
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Acid alcohol and about a dropper full and it is the decolorizer and 5 seconds.
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What is the fourth application of an acid-fast procedure and what is the purpose of the application and how long should the application be?
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Methylene blue and it is the counter stain and 30 seconds.
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