Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
51 Cards in this Set
- Front
- Back
|
viable count and serial dilution technique |
in this technique a series of dilutions are made from the culture and measured samples of these dilutions are made from the culture and measured samples of these dilutions are transferred to pour plates |
|
|
what are bacterial counts used to determine? |
potency of vaccines check safety in water supplies and raw foods |
|
|
scientific notation is used to simplify larger numbers in serial dilution technique |
know how to do scientific notation |
|
|
what can be done by health care workers to decrease the chance of cystitis during catheterization |
aseptic technique, hand washing |
|
|
what does tntc stand for |
to numerous to count |
|
|
write 1/100,000 in powers of ten |
1x10^-5 |
|
|
write 5,646,004 in scientific notation |
5.7x10^6 |
|
|
what three bacteria did we use for experiment 11 |
we used e coli pseudonomas and clostridium |
|
|
definition of aerobes is |
use oxygen as their final electron |
|
|
anaerobes |
killed by the presence of oxygen |
|
|
facultative anerobes |
can survive with or without oxygen |
|
|
microaerophiles |
do require oxygen however they grow best when the oxygen concentration is lower |
|
|
capnophiles |
microorganisms that require extra carbon dioxide |
|
|
why do we use a candle jar |
it is large sealed and as the candle burns it will use oxygen and create carbon dioxide |
|
|
E.coli |
facultative anaerobe cannot survive in the presence of O2 |
|
|
clostridum |
obligate anaerobe should be growing at the bottom |
|
|
pseudonomas |
obligate aerobe must have oxygen to live but grow at top necause O2 present |
|
|
why do aerobic organisms need oxygen |
oxygen acts as final electron |
|
|
is a candle jar anareobic |
no still oxygen present its an obligate anerobe |
|
|
what is the most abundant species of normal flora in the human colon |
e coli |
|
|
why does pseudonomas give negative reactions on all the fermentations |
it is an anaerobic organism therefore it does not ferment |
|
|
why must you have a pure culture for inncolutaion f sugar fermentation and other biochemical tests what would happen if the culture were contaminated or mixed |
because if its not a pure culture you would have a skewed results on what the test would give you because you wouldnt be testing one microorganism |
|
|
define IMViC |
Indo methyl red |
|
|
is MacConkey agar a differential medium a selective medium or both |
both |
|
|
what is the diffrence between alpha beta and gamma hemolysis |
beta is the complete clearing of the blood agar alpha is a mere discoloring of the blood agar and gamma is no change |
|
|
define coagulase |
an enzyme that clots things up |
|
|
name three pathogenic species of streptococcus and the disease caused by them |
streptococcus phenomia-phnemonia |
|
|
whats the diffrence between dry heat and moist heat |
moist heat is boiling water steam under pressure and dry heat is dry oven incineration etc moist heat is better |
|
|
differentiate between tdt and tdp |
tdt is thermal death time the time required to kill a particular species of microorganisms at a particular temperature and tdp is the lowest temperature required to kill all of a certain species of bacteria within a given amount of time |
|
|
why is pressure used in an autoclave |
because pressure increases the boiling point of water when it does boil the steam is not superheated |
|
|
uv light is |
form of radiation with wavelength hat is a little shorter than visible light the shorter wavelength causes the uv light to have more energy when certain molecules absorb this light chemical changes can take place |
|
|
abnormal linkages are called |
pyrimidine dimers |
|
|
the bonds that can occur between two adjacent thymines |
thymine dimers |
|
|
cytosine |
cytosine dimers |
|
|
uv light cant penetrate through |
glass or liquid or plastic |
|
|
give two examples of when uv light is used to control microorganisms |
to sterilize rooms, medical instruments and to stimulate the immune system |
|
|
define a thymine dimer |
chemically bonded adjacent thymine in DNA |
|
|
definition of disinfectants |
chemical agents that kill vegetative pathogenic microorganisms |
|
|
microbial cells are made of biological compounds such as proteins Dna lipids in order to destroy these compounds chemical agents will react with these compounds through coagulating oxidizing or in other ways |
|
|
|
what happens when a bacterias proteins are coagulated |
they can no longer function |
|
|
cidal |
chemicals that kill bacterial cells |
|
|
static |
chemical that inhibits the growth of bacteria |
|
|
definition of epidemiology |
the study of the spread of disease |
|
|
epedemiologists definition |
they are employed by local and state wide health departments the national center for disease control cdc and global world health organizations |
|
|
definition of prevelance |
referes to the total number of people in a population who have a diease at a [articular time regardless of when they caught it or when they were diagnosed |
|
|
definition of incidence |
measures the number of new cases of disease that occur within a certain time period |
|
|
definition of epidemic |
when a disease is spreading rapidly in a certain population |
|
|
definition of pandemic |
when a disease is spreading globally or possibly over the continent |
|
|
definition of endemic |
when the location is fairly stable |
|
|
definition of sporadic |
when it occurs infrequently and i scattered locations |
|
|
. |
good job |
